Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (40): 7479-7482.doi: 10.3969/j.issn.1673-8225.2010.40.015

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Effects of transplantation of X-box-binding protein-1 gene-modified neural stem cells on focal brain ischemia/reperfusion injury in rats

Jin Xian-hua1, Zheng Sheng-zhe1, Lu Lei2, Song Lei2   

  1. 1 Department of Neurology, Hospital Affiliated to Yanbian University, Yanji  133000, Jilin Province, China; 2 Department of Neurology, First Hospital, Jilin University, Changchun  130021, Jilin Province, China
  • Online:2010-10-01 Published:2010-10-01
  • About author:Jin Xian-hua★, Master, Attending physician, Department of Neurology, Hospital Affiliated to Yanbian University, Yanji 133000, Jilin Province, China neurology@139.com

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Abstract:

BACKGROUND: Neural stem cells (NSCs) transfected with X-box-binding protein-1 (Xbp-1) gene transplantation to the lesion site not only ensures the stability of the Xbp-1 gene, sustains effects of anti-apoptotic role, but also promotes survival and differentiation of NSCs following transplantation.
OBJECTIVE: To study the effects of Xbp1 gene on the distribution, differentiation and apoptosis of NSCs transplanted into rat brain ischemic injury site, the anti-apoptosis effect and recovery of nerve function.
METHODS: Adult Sprague Dawley rats were selected to establish models of middle cerebral artery occlusion (MCAO). The rats were randomly assigned to four groups: control group (no treatment), phosphate buffered saline (PBS) transplantation group, NSCs transplantation group, Xbp-1-NSCs transplantation group. At 7, 14 and 28 days following treatment, neurological severity score was scored. Brain tissue was obtained to prepare into sections. Immunofluorescence staining was used to observe survival and distribution of NSCs in the brain. On day 28 following transplantation, terminal deoxyribonucleotidyl transferase-mediated biotin- 16-dUTP nick-end labeling assay was used to determine apoptosis of neural cells in the ischemic region. Western blot assay was utilized to detect Bcl-2 expression levels.
RESULTS AND CONCLUSION: At 7, 14 and 28 days following transplantation, neurological severity score was significantly lower in the Xbp-1-NSC transplantation group compared with other three groups (P < 0.05). NSCs could successfully migrate to the ischemic regions, survived and differentiated into mature nerve cells. Survival, proliferation and differentiation ability of Xbp-1 gene-modified NSCs was stronger than the common NSCs (P < 0.05). Compared with other three groups, apoptotic number of NSCs in the ischemic regions was significantly reduced, but Bcl-2 levels increased in the Xbp-1-NSCs transplantation group (P < 0.05). These verified that Xbp-1 gene modification can increase NSCs survival and migration ability, obviously decrease neurological severity score in rat models after transplantation by anti-endoplasmic reticulum stress, as well as promote the recovery of neurological function.

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