Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (36): 6744-6750.doi: 10.3969/j.issn.1673-8225.2010.36.021

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Effects of bone marrow mesenchymal stem cells transplantation on alpha-smooth muscle actin expression in acute radiation-induced liver injury rats

Ji Wei-zheng, Ma Yan, Wen Hao   

  1. First Affiliated Hospital, Xinjiang Medical University, Urumqi  830054, Xinjiang Uygur Autonomous Region, China
  • Online:2010-09-03 Published:2010-09-03
  • Contact: Wen Hao, Doctor, Chief physician, Professor, Doctoral supervisor, First Affiliated Hospital, Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China
  • About author:Ji Wei-zheng☆, Studying for doctorate, Associate chief physician, First Affiliated Hospital, Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China jwzgxl@sina.com
  • Supported by:

    the Stem Cell Special-Purpose Foundation of the First Affiliated Hospital of  Xinjiang Medical University, No.2009GXB02*

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Abstract:

BACKGROUND: Radiation-induced liver injury has greatly limited the clinical application of radiotherapy. Research demonstrated that liver injury environment is suitable for the differentiation of bone marrow mesenchymal stem cells (BMSCs), which suggested that it is possible to treat radiation-induced liver injury with BMSCs transplantation.
OBJECTIVE: To test effect of BMSCs transplantation on ɑ-smooth muscle actin (SMA) expression level in acute radiation-induced liver injury rats.
METHODS: BMSCs were isolated from male rats, and subcultured. The third passage of BMSCs was selected as transplanted cells. Female Sprague Dawley rat models of acute radiation-induced liver injury were established, and randomly assigned to two groups. Within 4 hours following radiotherapy, BMSCs suspension was infused through caudal vein in the intervention group. An equal volume of saline was infused into the control group. ɑ-SMA expression was detected by immunohistochemistry in the liver. Sex determining gene Y (SrY) was determined using in situ hybridization technique. Pathomorphological change in the liver was observed under an optical microscope.
RESULTS AND CONCLUSION: The positive expression rate of ɑ-SMA in right lobe of liver of intervention group was lower than control group (P < 0.05). The existence of SrY was more in the right lobe of liver than that of in the left lobe of liver in intervention group(P < 0.05). In control group, no SrY gene can be found. The injury degree of right lobe liver in intervention group was lower than control group. Results have verified that acute radiation-induced liver injury can lead BMSCs’ homing in male rats. BMSCs can down-regulate the expression level of ɑ-SMA induced by acute radiation in liver injury rats.

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