Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (36): 6738-6743.doi: 10.3969/j.issn.1673-8225.2010.36.020

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Effects of bone marrow mesenchymal stem cell transplantation on growth-associated protein-43 expression and infarction volume following permanent middle cerebral artery occlusion in rats

Zhang Qi-mei1, Peng Yu1, Zhuang Wei-hua1, Lan Jing1, Li Cheng-yan2   

  1. 1 Department of Neurology, Yichang Central People’s Hospital, First Clinical College, China Three Gorges University, Yichang  443003, Hubei Province, China; 2 Department of Neurology, Renmin Hospital, Wuhan University, Wuhan  430060, Hubei Province, China
  • Online:2010-09-03 Published:2010-09-03
  • Contact: Peng Yu, Department of Neurology, Yichang Central People’s Hospital, First Clinical College, China Three Gorges University, Yichang 443003, Hubei Province, China ycpengyu@126.com
  • About author:Zhang Qi-mei★, Master, Chief physician, Associate professor, Department of Neurology, Yichang Central People’s Hospital, First Clinical College, China Three Gorges University, Yichang 443003, Hubei Province, China

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Abstract:

BACKGROUND: There are numerous studies on bone marrow mesenchymal stem cells (BMSCs) transplanted in the brains of ischemia rats survive and migrate through blood-brain barrier, partially differentiate into neurons, promote the secretion of various neurotrophic factors, significantly improve neurological impairment, and have long therapeutic time window.
OBJECTIVE: To explore the effect of BMSC transplantation on the expression of growth-associated protein-43 and the infarct volume following permanent middle cerebral artery occlusion in rats.
METHODS: Adult Sprague-Dawley rats were randomly assigned to model control group, sham operation group and stem cell transplantation group. An additional four adult Sprague Dawley rats were used to prepare BMSCs. BMSCs were labeled with 5-bromodeoxyuridine. Rats in the sham operation group were utilized to deligate right common carotid artery. The remaining rats were employed to establish right middle cerebral artery models. Following model induction, BMSCs were infused into the rats from the stem cell transplantation group. An equal volume of phosphate buffer saline was injected into the rats in the model control group. Neurological impairment was graded prior to transplantation, at 7, 14, 21 and 28 days following transplantation. Expression of growth associated protein-43 was determined surrounding the infarct region using the immunohistochemistry.
RESULTS AND CONCLUSION: In the stem cell transplantation group, 5-bromodeoxyuridine-positive cells were detected in the infarct site at 7 day following transplantation, peaked at 14 days, reduced and disappeared at 28 days. Immunological activity of growth associated protein-43 was significantly higher in the stem cell transplantation group than in the model control group at 7 and 14 days following transplantation (P < 0.05). No nerve injury was tested in rats from the sham operation group, and the score of neural function was 0 point. With time prolonged, the score of neural function was gradually reduced in the model control and stem cell transplantation groups. The score was significantly lower in the stem cell transplantation group than in the model control group at 14 days following transplantation (P < 0.05). Compared with model control group, cerebral infarct volume was significantly diminished in the stem cell transplantation group (P < 0.05). Results indicated that BMSC transplantation can upregulate growth associated protein-43 expressions surrounding the infarct region after focal cerebral ischemia, can significantly decrease infarct volume.

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