Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (36): 6724-6728.doi: 10.3969/j.issn.1673-8225.2010.36.017

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Apoptotic protease activating factor-1 expression in the heart, liver and lung of rabbits with multiple organ dysfunction syndrome following bone marrow mesenchymal stem cells transplantation

Ling Bin1, Chen Jing1, Wang Ting-hua2, Pan Xing-hua3, Sun Jie1, Liu Ping1   

  1. 1 Department of ICU, Second People’s Hospital, Kunming  650031 ,Yunnan Province, China; 2 Institute of Neuroscience, Kunming Medical College, Kunming  650031, Yunnan Province, China; 3 Department of Clinical Trials, Kunming General Hospital of Chengdu Military Area Command of Chinese PLA, Kunming  650031, Yunnan Province, China
  • Online:2010-09-03 Published:2010-09-03
  • About author:Ling Bin, Master, Chief physician, Master’s supervisor, Department of ICU, Second People’s Hospital, Kunming 650031, Yunnan Province, China Lingbin02@yahoo.com
  • Supported by:

    the   Natural Science      Foundation of Yunnan Province, No.  2007C249M

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Abstract:

BACKGROUND: The multiple organ dysfunction syndrome (MODS) onset and organ tissue injury are associated with cell apoptosis and necrosis. However, bone marrow mesenchymal stem cells (BMSCs) as seed cells are effective in repair field.
OBJECTIVE: To observe BMSCs transplantation effects on apoptotic protease-activating factor 1 (Apaf-1) expression in involved organs of rabbits with MODS.
METHODS: Two-hit animal model of MODS was established in the experimental group. Rabbit BMSCs were cultured, amplified and identified in vitro, and then labeled with GFP by lentivirus. BMSCs were transplanted via the femoral artery. We also set model control and normal groups. The survival of BMSCs in model of MODS was observed. Apaf-1 gene expression was detected in the heart, liver and lung prior to and following transplantation using RT-PCR.
RESULTS AND CONCLUSION: Lentiviral transgenic GFP marker presence of BMSCs was visible in the rabbit liver and lung in the experimental group. RT-PCR results showed that Apaf-1 mRNA expression was determined in the heart, liver and lung tissues of normal group, and significantly increased in the heart, spleen and kidney of model control group. Apaf-1 mRNA expression was decreased in the rabbit heart, spleen and kidney of experimental group. These indicated that BMSCs have good biological activity. Transplanted BMSCs could implant in the damaged organs of rabbits with MODS, downregulate Apaf-1 mRNA expression, which suggested that BMSCs transplantation can provide a new therapeutic method for MODS.

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