Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (36): 6667-6671.doi: 10.3969/j.issn.1673-8225.2010.36.004

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Bone marrow mesenchymal stem cells-induced transformation of cardiac fibroblasts into myofibroblasts: Optimal microenvironment of myocardial infarction at different stages

Lin Chu-wei, Zhou Sheng-hua, Liu Qi-ming, Du You-you, Yao Li-qun, Zhang Liang, Cai Xiang-ping   

  1. Department of Cardiology, Second Xiangya Hospital, Central South University, Changsha  410000, Hunan Province, China
  • Online:2010-09-03 Published:2010-09-03
  • About author:Lin Chu-wei☆, Studying for doctorate, Department of Cardiology, Second Xiangya Hospital, Central South University, Changsha 410000, Hunan Province, China linchuwei@yahoo.com.cn
  • Supported by:

    the National Natural Science Foundation of China, No. 30871053*; the Natural Science Foundation of Hunan Province, No. 10JJ3064*

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Abstract:

BACKGROUND: Bone marrow mesenchymal stem cells (BMSCs) can effectively improve heart function following myocardial infarction. Its action mechanism is associated with aggregation of a large number of myofibroblasts in the transplant region, but few reports have verified that gathered myofibroblasts are transformed from BMSCs or from fibroblasts.
OBJECTIVE: To explore the impact of microenvironment of myocardial infarction in different periods in rat BMSCs inducing cardiac fibroblasts into myofibroblasts.
METHODS: The culture group was assigned to three groups: rat BMSCs group, cardiac fibroblasts group and combination group. Myocardial homogenate was added at different times after myocardial infarction for 7-28 days. A blank control group was set, without myocardial homogenate. Immunocytochemical staining was used to determine the differences of α-smooth muscle actin (α-SMA) expression and the number of fibroblasts into myofibroblasts between the two groups.
RESULTS AND CONCLUSION: In the combination group, the positive rate of cardiac fibroblasts into myofibroblasts is highest, when BMSCs and cardiac fibroblasts were cultured together and intervened by myocardial homogenate on day 14 after myocardial infarction. Results suggest that in the microenvironment of post-myocardial infarction, BMSCs can induce cardiac fibroblasts into myofibroblasts, while the microenvironment of post-myocardial infarction on day 14 is the best one.

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