Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (27): 5067-5070.doi: 10.3969/j.issn.1673-8225.2010.27.029

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Isolation, culture and identification of adipose tissue-derived mesenchymal stem cells

Yao Su-yan1, Yang Yi-yong2, Qin Shu-jian2, Zheng De-yu2   

  1. 1 Department of Pathophysiology, 2 Department of Anatomy, Liaoning Medical University, Jinzhou  121001, Liaoning Province, China
  • Online:2010-07-02 Published:2010-07-02
  • Contact: Zheng De-yu, Associate professor, Department of Anatomy, Liaoning Medical University, Jinzhou 121001, Liaoning Province, China zheng_deyu2000@yahoo.com.cn
  • About author:Yao Su-yan★, Master, Associate professor, Department of Pathophysiology, Liaoning Medical University, Jinzhou 121001, Liaoning Province, China ysyzdy@yahoo.com.cn
  • Supported by:

    the Innovation Team Program of Department of Education of Liaoning Province, No. 2006T063*

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Abstract:

BACKGROUND: Previous studies have demonstrated that there are similar mesenchymal stem cells in adipose tissue as bone marrow mesenchymal stem cells (BMSCs). Adipose tissue can be obtained from lipid absorption of cosmetology, and is characterized by abundant source and convenient collection, which shows great value in clinical application.
OBJECTIVE: To isolate and culture human adipose tissue-derived mesenchymal stem cells (ADMSCs) and to measure its biological properties.
METHODS: Human ADMSCs were isolated by centrifugation and cultured in DMEM medium containing 10% fetal bovine serum. The morphology of cells was observed, and cell growth curve was drawn. Cell surface antigens were measured using flow cytometry.
RESULTS AND CONCLUSION: ADMSCs obtained by digestion and centrifugation were similar in size, spindle-shaped or star-shaped fibroblasts-liked cells. Cell growth curve analysis indicated that ADMSCs were in the exponential stage at 5 days following incubation. The number of ADMSCs was reduced at 9 days. Flow cytometry analysis showed that more than 90% cells were positive for CD29, CD45 and CD105. These indicated that human ADMSCs isolated in vitro grew stably and can be used as tissue engineered seed cells.

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