Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (27): 5018-5022.doi: 10.3969/j.issn.1673-8225.2010.27.018

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Effects of cardiac tissue mass cultured ex vivo on migration of mesenchymal stem cells in myocardial infarction rats

Wang Ding-qiong1, Shi Ruo-fei2, Zhang Xiao-gang1, Liao Li-qiang1   

  1. 1 Department of Cardiology, First Affiliated Hospital, Chongqing Medical University, Qiongqing  400016, China; 2 Chongqing Emergency Medical Center, Chongqing  400014, China
  • Online:2010-07-02 Published:2010-07-02
  • Contact: Zhang Xiao-gang, Doctor, Professor, Chief physician, Master’s supervisor, Department of Cardiology, First Affiliated Hospital, Chongqing Medical University, Qiongqing 400016, China zxg0233@sina.com
  • About author: Wang Ding-qiong★, Studying for master’s degree, Physician, Department of Cardiology, First Affiliated Hospital, Chongqing Medical University, Qiongqing 400016, China dingding1984@163. com

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Abstract:

BACKGROUND: Mesenchymal stem cells (MSCs) have the potential to improve cardiac function, but the mechanisms of MSCs homing to heart post-implantation are not completely clear.
OBJECTIVE: To investigate the role and possible mechanisms of cardiac tissue mass cultured ex vivo which isolated from myocardial infarction rats on MSC migration. 
METHODS: Rat models of myocardial infarction were established and cardiac tissue masses were cultured. MSCs were isolated from bone marrow by density gradient centrifugation and adherence screening method. A total of 24 Sprague Dawley rats were randomly assigned to myocardial infarction, sham operation and normal control groups. The rats in the sham operation group were only threaded without deligation. The rats in the normal control group were left intact. MSCs were labeled with DAPI. A transwell model was used to co-culture for 48 hours. The number of migrating cells was counted under a fluorescence microscope. Quantitative cell count was conducted for CD34/CD44 on MSCs that were tested using immunocytochemistry staining under a fluorescence microscope. Immunofluorescent staining was used to evaluate CXCR4 expression on MSCs. Immunohistochemistry staining was used to detect expression of stromal cell-derived factor-1 on cardiac sections and mean absorbance was analyzed.
RESULTS AND CONCLUSION: Number of migrating MSCs in myocardial infarction group was significantly greater compared with sham operation group (P < 0.05). No migrating cells were detectable in the normal control group. Immunocytochemical staining results have shown that migrating cells were positive for CD44, but negative for CD34, which were consistent with MSC characteristics. The membrane receptor CXCR4 was positively expressed on the migrating cells. Stromal cell-derived factor-1-positive expression was observed on cardiac tissue sections both in myocardial infarction and sham operation groups, rather than in normal control group. Mean absorbance of stromal cell-derived factor-1 was significantly higher in the myocardial infarction group than in the other groups (P < 0.05). Results have indicated that cardiac tissue post myocardial infarction can promote migration of MSCs, which might be associated with effects of stromal cell-derived factor-1-CXCR4 axis.

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