Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (27): 4970-4974.doi: 10.3969/j.issn.1673-8225.2010.27.007

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Screening of optimal labeling time and labeling concentration of rabbit bone marrow mesenchymal stem cells by 5-bromodeoxyuridine in vitro

Zhu Feng1, Guo Guang-hua2, Zhan Jian-hua2, Xie An3   

  1. 1 Department of Critical Care Medicine, 2 Department of Burn, 3Urology Institute, First Affiliated Hospital, Nanchang University, Nanchang  330006, Jiangxi Province, China
  • Online:2010-07-02 Published:2010-07-02
  • Contact: Guo Guang-hua, Chief physician, Doctoral supervisor, Department of Burn, First Affiliated Hospital, Nanchang University, Nanchang 330006, Jiangxi Province, China guogh2000@hotmail.com
  • About author:Zhu Feng☆, Studying for doctorate, Lecturer, Attending physician, Department of Critical Care Medicine, First Affiliated Hospital, Nanchang University, Nanchang 330006, Jiangxi Province, China zhujunchi@tom.com
  • Supported by:

    the Youth Science Foundation of Department of Education of Jiangxi Province, No.GJJ09432*

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Abstract:

BACKGROUND: Currently, bone marrow mesenchymal stem cells (BMSCs) are potential cells and host cells for gene therapy. Labeling in vitro is a premise to track their survival, “homing”, growth, differentiation and so on.
OBJECTIVE: To explore the optimal 5-bromodeoxyuridine (BrdU) concentration and time of labeling for rabbit BMSCs in vitro.
METHODS: Rabbit BMSCs were harvested from bone marrow of purebred healthy New Zealand white rabbits. BMSCs were identified by flow cytometry and morphology. Rabbit BMSCs-labeling positive rates using BrdU at different concentrations and different incubation time were observed.
RESULTS AND CONCLUSION: Flow cytometry finally confirmed that the cells we cultured were BMSCs combined with morphological observation; the growth curve of rabbit MSCs was “S” shape. The positive rate of BMSCs labeled by BrdU increased with incubation time and BrdU concentration, and more than 85%-95% BMSCs were labeled after at optimal 40 μmol/L and 72 hours. Above-mentioned results have suggested that application of BrdU labeling for BMSCs in vitro is safe and reliable. The optimal time and concentration for labeling BMSCs are 72 hours and 40 μmol/L.

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