Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (18): 3335-3338.doi: 10.3969/j.issn.1673-8225.2010.18.025

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Expression of Tpap gene in mouse testis

Tang Ai-fa, Yu Zhou, Zhang Xiao-yan, Zhang Zhen-ming, Gui Yao-ting, Ye Jiong-xian, Cai Zhi-ming   

  1. Guangdong Provincial Key Laboratory of Male Reproduction and Genetics, Shenzhen Hospital, Peking University, Shenzhen  518036, Guangdong Province, China
  • Online:2010-04-30 Published:2010-04-30
  • Contact: Cai Zhi-ming, Guangdong Provincial Key Laboratory of Male Reproduction and Genetics, Shenzhen Hospital, Peking University, Shenzhen 518036, Guangdong Province, China caizhiming2000@yahoo.com.cn
  • About author:Tang Ai-fa☆, Doctor, Associate researcher, Guangdong Provincial Key Laboratory of Male Reproduction and Genetics, Shenzhen Hospital, Peking University, Shenzhen 518036, Guangdong Province, China tangaifa2004@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 3070082*, 30972992*; “973” Program, No. 2008CB517412*; Doctoral Fund of Higher Education of China, No. 200800010106*; Natural Science Foundation of Guangdong Province, No. 06027982*; Medical Scientific Research Foundation of Guangdong Province, No. A2008634*; Supporting Projects of Provincial Subjects, No. PT200805200232A*

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Abstract:

BACKGROUND: These serial processes for forming male gametes are basically controlled by the programmed expression of a number of stage-specific genes. However, many aspects of the mechanisms of spermatogenesis have remained elusive because of a lack of suitable in vitro or in vivo models.
OBJECTIVE: To screen genes involved in spermatogenesis, and to analyze its expression characteristics. 
METHODS: Testes cDNA samples from Balb/C mice of different postnatal days (4, 9, 18, 35, 54 days and 6 months, respectively) were hybridized with mouse whole genome Affymetrix chip to screen the testis-ralated genes. The characteristics of the selected genes were analyzed by various bioinformatics tools. RT-PCR was used here to identify the expression of the selected genes in mice testis. 
RESULTS AND CONCLUSION: The Affymetrix chip probe of mouse Tpap was graduated higher expression with developmental stages of mouse testis. The scaling hybridization signal intensities of the tested testis on days 4, 9, 18, 35, 54, and 6 months of postnatal were 4.4 (Absent expression, A), 12.9 (A), 262.4 (Present expression, P), 1136.7 (P), 1617.5 (P) and 1128 (P), respectively. These results indicated that the expression of mouse Tpap wasn’t detected on days 4 and 9, but was detected on days 18, 35, 54, and 6 months of mouse testis in our Affymetrix chip analysis. By combination with the RT-PCR analysis of mouse Tpap, we observed mouse Tpap began to express at the age of day 18 in mouse. Tpap is an age-dependent gene in mouse testis. The expression of Tpap corresponds to the appearance of spermatids of mice and indicates that Tpap may have an important role in male mammalian spermatogenesis.

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