Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (18): 3310-3313.doi: 10.3969/j.issn.1673-8225.2010.18.019

Previous Articles     Next Articles

Swim-up and density gradient centrifugation preparation techniques for intrauterine insemination: A systematic review

Li Tao 1，2， Guo Qing-hua3,Tian Jin-hui4, Zhang Wei3, Guo Bai-hong5, Li Guo-ping2, Luo Neng-qin5, Li Zhao-bin1, Jiang Lei 3, 4, Jia Wen-qin4, Li Ren-ju2, Zhang Peng 2 , 4, Chen Yi-rong5   

  1. 1 Department of Andrology, Woman and Child Health Hospital, Baoji   721000, Shaanxi Province, China;    2 First Clinical Medical College of Lanzhou University, Lanzhou  730000, Gansu Province, China; 3 First Hospital of Lanzhou University, Lanzhou  730000, Gansu Province, China;4 Evidence-
    based Medicine Center of Lanzhou University, Lanzhou  730000, Gansu Province, China; 5 People’s Hospital of Gansu Province, Lanzhou   730000, Gansu Province, China
  • Online:2010-04-30 Published:2010-04-30
  • Contact: Chen Yi-rong, Master, Chief physician, Doctoral supervisor, People’s Hospital of Gansu Province, Lanzhou 730000, Gansu Province, China cyirong@yahoo.com
  • About author:Li Tao★, Studying for master’s degree, Attending physician, Department of Andrology, Woman and Child Health Hospital, Baoji 721000, Shaanxi Province, China; 2First Clinical Medical College of Lanzhou University, Lanzhou 730000, Gansu Province, China litao22222828@sina.com

PDF

504

Abstract:

BACKGROUND: There are many in vitro selection method of sperm, and swim-up and density gradient centrifugation are commonly used. It remains unclear which method minimizes bad stimulation to the sperm and select sperm with high fertilization potential.
OBJECTIVE: To evaluate the effectiveness of swim-up and gradient centrifugation preparation techniques on intrauterine insemination (IUI).
METHODS: A computer-based online search of Cochrane Library, PubMed, EMBASE databases was performed, and some related journals were manually searched for related articles published between January 1966 and February 2009. The quality of included randomized controlled trials (RCT) and q-randomized trials (Q-RCT) was evaluated and Meta-analysis was conducted by the Cochrane Collaboration’s software RevMan5.0. Experts.
RESULTS AND CONCLUSION: A total of 6 studies were included, involving 4 RCTs and 2 Q-RCTs. A total of 486 patients     (1 099 IUI cycles) were enrolled. The Meta-analysis indicated that there was no difference between swim-up and gradient centrifugation preparation techniques for the IUI in terms of cycle pregnancy rates [OR = 1.11, 95%CI (0.8, 1.55)], miscarriage rates [OR = 0.31, 95%CI (0.09,1.04)], sperm count [the weight mean difference (WMD) = -0.89, 95%CI(-14.17, 12.38)], sperm motility [WMD = -2.31, 95%CI(-7.27, 2.65)]. There is insufficient evidence to confirm which is the best method in the two specific preparation techniques. The quality of study methods should be improved. And more measure parameters should be included when comparing it before or after treatment, such as sperm motility, sperm count, sperm function.

CLC Number: