Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (14): 2617-2620.doi: 10.3969/j.issn.1673-8225.2010.14.030

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Primary culture of spiral ganglion cells from cochlea of neonatal C57 mice 

Wang Ting-kuo, Sun Hong   

  1. Department of Otorhinolaryngology-Head ＆ Neck Surgery, Third Xiangya Hospital, Central South University, Changsha   410013, Hunan Province, China
  • Online:2010-04-02 Published:2010-04-02
  • Contact: Sun Hong, Professor, Department of Otorhinolaryngology-Head ＆ Neck Surgery, Third Xiangya Hospital, Central South University, Changsha 410013, Hunan Province, China shjhaj@vip163.com
  • About author:Wang Ting-kuo☆, Studying for doctorate, Attending physician, Department of Otorhinolaryngology-Head ＆ Neck Surgery, Third Xiangya Hospital, Central South University, Changsha 410013, Hunan Province, China wtk711112@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30371531*，30772402*; the Natural Science Foundation of Hunan Province, No. 02JJY2050*; the Scientific Research Foundation of Health Department of Hunan Province, No. B2006-063*; the Key Program of Science and Technology Project of Department of Science and Technology of Hunan Province, No. 2007SK2001*

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Abstract:

BACKGROUND: At present, there are differences in reporting culture condition of cochleal spiral ganglion cells (SGCs) in circle of primary cell culture. Individual method has poor repeatability, and is not beneficial for practical application.
OBJECTIVE: To primary culture and evaluate SGCs of neonatal C57 mice.
METHODS: Tissues from cochlear modiolus were acquired from neonatal C57 mice by microanatomy. SGCs from cochlear modiolus were cultured by trypsin digestion, differential velocity adherent technique combined with chemicals. Cell growth was observed by inverted phase contrast microscope and hematoxylin and eosin staining. Immunocytochemistry was employed to classify cell types. 
RESULTS AND CONCLUSION: After purification, the cell body of SGCs from cochlear modiolus was ellipse or triangle, with slender processes in cytoplasm. Nuclei were positive for Nuen (stained brown). Cytoplasm and axon were positive for β3-Tubulin (stained brown). These indicated that mouse SGCs were successfully cultured.

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