Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (14): 2583-2587.doi: 10.3969/j.issn.1673-8225.2010.14.023

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Neuroprotective effect of glial cell line-derived neurotrophic factor gene-modified bone marrow stromal cells transplantation on intracerebral hemorrhage in rats 

Deng Li1, Yang Chao-xian1, Tu Jiang-yi2, Gao Xiao-qing1, Guo Kan1, Yuan Qiong-lan3   

  1. 1 Department of Neurobiology, 2 Department of Anatomy, Luzhou Medical College, Luzhou  646000, Sichuan Province, China; 3 Department of Anatomy and Neurobiology, Tongji University School of Medicine, Shanghai   200092, China
  • Online:2010-04-02 Published:2010-04-02
  • Contact: Yang Chao-xian, Professor, Master’s supervisor, Department of Neurobiology, Luzhou Medical College, Luzhou 646000, Sichuan Province, China yangchaoxian2006@tom.com
  • About author:Deng Li★, Master, Lecturer, Department of Neurobiology, Luzhou Medical College, Luzhou 646000, Sichuan Province, China dy9611@163.com

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Abstract:

BACKGROUND: Previous studies have demonstrated that cell transplantation has neuroprotective effect on intracerebral hemorrhage, and some researches have indicated that transplantation of bone marrow stromal cells (BMSCs) can promote neural function recovery after cerebral infarction.
OBJECTIVE: To explore whether transplantation of BMSCs-modified by glial cell line-derived neurotrophic factor gene (GDNF) gene provides a better therapeutic effect than native BMSCs after stroke.
METHODS: Totally 36 SD rats were induced intracerebral hemorrhage models by injecting autologous arterial blood, and then divided into 3 groups (n = 6), each group was assigned into 2 sub-groups. Rabbits in each group were stereotaxically grafted with 20 μL GDNF/BMSCs, BMSCs or saline respectively. The rats were executed at 1 and 2 weeks after operation, and immunohistochemistry was used to observe the expressions of synaptophysin (Syn) and growth associated protein-43 (GAP-43) in the margin of the hemorrhagic focus.
RESULTS AND CONCLUSION: Compared with the BMSCs and control groups, both Syn-immunoreactive and GAP-43-immunoreactive products were significantly increased in the GDNF/BMSCs group (P < 0.05). Present results demonstrate that transplantation of GDNF gene-modified BMSCs provides better neuroprotection than native BMSCs delivery for stroke.

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