Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (14): 2485-2491.doi: 10.3969/j.issn.1673-8225.2010.14.004

Previous Articles     Next Articles

Immunomodulatory effect of Wharton’s jelly-derived mesenchymal stem cells from human umbilical cord on human peripheral blood T lymphocytes  

Zhou Chang-hui 1, 2, Tian Yi 2, 3, Yang Bo 2, 3, Hu Xiang 4, 5, Jiao Hong-liang 2, Zhou Yun-fan1, Wang Cheng-chun1, Gu Chen-xi6, Lei Ning-jing1, Guan Fang-xia1   

  1. 1 Department of Bioengineering, Zhengzhou University, Zhengzhou  450001, Henan Province, China; 2 Open Laboratory of Clinical Medicine and Key Subject, Henan Institution of Higher Education, Zhengzhou   450052, Henan Province, China; 3 Department of Neurosurgery, First Affiliated Hospital, Zhengzhou University, Zhengzhou   450052, Henan Province, China; 4 Jiangsu Public Technology Service Platform of Stem Cells and Biotherapy, Taizhou  225300, Jiangsu Province, China; 5 Shenzhen Beike Cell Engineering Institute, Shenzhen  518000, Guangdong Province, China; 6Department of Clinical Medicine, Zhengzhou University, Zhengzhou  450001, Henan Province, China
  • Online:2010-04-02 Published:2010-04-02
  • Contact: Guan Fang-xia, Doctor, Professor, Department of Bioengineering, Zhengzhou University, Zhengzhou 450001, Henan Province, China guanfangxia@126.com
  • About author:Zhou Chang-hui★, Studying for master’s degree, Department of Bioengineering, Zhengzhou University, Zhengzhou 450001, Henan Province, China; Open Laboratory of Clinical Medicine and Key Subject, Henan Institution of Higher Education, Zhengzhou 450052, Henan Province, China zhouchanghui008@163.com
  • Supported by:

    the 211 Project-phase Ⅲ of Zhengzhou University*; the Developmental Project from Science and Technology of Department of Science and Technology of Henan Province in 2009, No. 092102310250*; the Erqi District Society Development Project from Bureau of Science and Technology in Zhengzhou City, No.20095157*; the Public Technology Service Platform of Stem Cells and Biotherapy of Jiangsu Province, No. BM2008146*

PDF

384

Abstract:

BACKGROUND: Bone marrow mesenchymal stem cells have low immunogenicity and immunomodulatory effect, but there are seldom reports concerning the immunomodulatory effect of Wharton’s jelly-derived mesenchymal stem cells of human umbilical cord and its mechanims.
OBJECTIVE: To investigate the immunomodulatory effects and mechanisms of Wharton’s jelly-derived mesenchymal stem cells of human umbilical cord on varient peripheral blood T lymphocytes.
METHODS: Mesenchymal stem cells were isolateded from Wharton’s jelly of human umbilical cord by tissue culture. T lymphocytes from human peripheral blood were stimulated by phytohemagglutinin and co-cultured with umbilical cord Wharton’s jelly-derived mesenchymal stem cells and umbilical cord Wharton’s jelly-derived mesenchymal stem cells supernatant respectively to measure A value following 72 hours of coculture using multifunctional microplate reader. Expression of cytokines including transforming growth factor-beta 1 (TGF-β1) and interferon-γ (IFN-γ) was evaluated by enzyme-labeled immunosorbent assay.
RESULTS AND CONCLUSION: Wharton’s jelly-derived mesenchymal stem cells could inhibite the proliferation of T lymphocytes induced by phytohemagglutinin. The proliferation inhibition rate was 56% (P < 0.01). Wharton’s jelly-derived mesenchymal stem cells supernatant also had inhibitory effects on proliferation of T lymphocytes induced by phytohemagglutinin, in a dose-dependent fashion. The proliferation inhibition rates were 8.3% and 27% respectively in the 50% Wharton’s jelly-derived mesenchymal stem cells supernatant and 100% Wharton’s jelly-derived mesenchymal stem cells supernatant groups (P < 0.05). Wharton’s jelly-derived mesenchymal stem cells significantly decreased γ-interferon secrted from T-lymphocytes (P < 0.05). The secretion of TGF-β1 was lower in the coculture of Wharton’s jelly-derived mesenchymal stem cells and T lymphocytes group than Wharton’s jelly-derived mesenchymal stem cells alone group (P < 0.05). These indicated that Wharton’s jelly-derived mesenchymal stem cells and Wharton’s jelly-derived mesenchymal stem cells supernatant have inhibitory effects on proliferation of T lymphocytes induced by phytohemagglutinin. The mechanims may be associated with cell contant and inhibition of γ-interferon secrted from T-lymphocytes.

CLC Number: