Abstract:

BACKGROUND: In oral tissue engineering, appearance of maxillofacial bone is complex and irregular, and maxillofacial bone supports the face, and highly correlates to this appearance. Therefore, in addition to restoring the function, appearance restoration is also important. Precise appearance restoration would become developing trends.
OBJECTIVE: To explore the effect of tissue-engineered bone constructed by hyaluronic acid in combination with recombinant bone morphogenetic protein (rhBMP-2)-induced bone marrow stromal stem cells (MSCs) in vitro on repairing maxillary alveolar ridge defect, and investigate the feasibility to maintain moulding by personalized template for the external fixation of titanium.
METHODS: Rabbit MSCs were cultured in vitro for bone induction to construct tissue-engineered bone in combination with sodium hyaluronate and rhBMP-2. The bone was implanted into the autologous alveolar ridge defect, plus personalized titanium template. After 4, 8, 12 weeks, animals were sacrificed and respectively subjected to radiation, conventional histological examination to observe in vivo osteogenesis of this tissue-engineered bone. In addition, rats implanted with autogenous bone fragments served as control.
RESULTS AND CONCLUSION: There were significant differences in gray scale between experimental and control groups at the first 4 weeks (P < 0.05). But at 8 and 12 weeks, no differences were observed (P > 0.05). In addition, there were significant differences in gray scale of newly-generated bone between 4 and 8 weeks and 8 and 12 weeks after operation (P < 0.05). Histological staining showed that osteogenesis of the experimental group was less than the control group at 4 weeks, and became similar at 8 weeks. Results show that the tissue-engineered bone constructed with hyaluronic acid sodium and rhBMP2F-induced MSCs displayed obvious osteogenesis, and personalized shaping titanium barriers play a role in the template to promote the new bone generated by a particular shape.