Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (11): 2081-2085.doi: 10.3969/j.issn.1673-8225.2010.11.045

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Gene expression profile in osteoblastic differentiation of bone marrow stromal cells stimulated by simvastatin Gene chip analysis

Meng Ya-qiang1, Zhang Liu1, Tian Fa-ming1,2, Han Da-cheng1, Zheng Jie1, Cai Jun1   

  1. 1Department of Orthopaedic Surgery, Affiliated Hospital of North China Coal Medical University, Tangshan  063000, Hebei Province, China;
    2Hebei Medical University, Shijiazhuang 050017, Hebei Province, China
  • Online:2010-03-12 Published:2010-03-12
  • Contact: Zhang Liu, Doctor, Professor, Doctoral supervisor, Department of Orthopaedic Surgery, Affiliated Hospital of North China Coal Medical University, Tangshan 063000, Hebei Province, China zhliu130@sohu.com
  • About author:Meng Ya-qiang, Master, Physician, Department of Orthopaedic Surgery, Affiliated Hospital of North China Coal Medical University, Tangshan 063000, Hebei Province, China
  • Supported by:

    the Natural Science Foundation of Hebei Province, No.C2006000580*

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Abstract:

BACKGROUND: Simvastatin enhanced the expression of bone morphogenetic protein-2 (BMP-2), which plays an anabolic role in bone metabolism and osteoblastic lineage differentiation. However, little is known about the molecular mechanism of simvastatin on regulation of bone marrow stromal cells differentiation. 
OBJECTIVE: To investigated the effect of simvastatin on osteoblastic differentiation of bone marrow stromal cells based on genetics level.
METHODS: Bone marrow stromal cells derived from femur and tibia were cultured in different mediums with simvastatin or vehicle for 7 days. Following extraction and purification, mRNA was reverse-transcripted into cDNA. Fluorescence labeling was employed and the samples were then hybridized with oligonucleotide chip to screen the different genes, which were utilized to analyze osteogenesis-related factors. Alkaline phosphatase and Von Kossa staining were performed at days 14 and 21, respectively.
RESULTS AND CONCLUSIONS: At day 14, alkaline phosphatase-positive cells were more in the experimental group than control group. Von Kossa staining demonstrated that simvastatin could promote BMSCs osteoblastic differentiation and mineralization. Comparative analysis showed that 103 genes out of 22 575 rat genes had differential expression (≥ 2 fold or ≤ 0.5 fold), and some genes were related to cell proliferation and ostoeblastic differentiation, including C/EBPδ, Cited, Ascl2, Ptpn16, Wisp2, Tieg, etc. Simvastatin could induce osteoblastic differentiation of bone marrow stromal cells, involving in many osteogenetic-related genes.

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