Abstract:

OBJECTIVE: Studies have confirmed that basic fibroblast growth factor stimulating periodontal ligament cells can promote the proliferation of human periodontal ligament cells, so as to facilitate the reconstruction of lost periodontal tissues. By use of different concentrations of basic fibroblast growth factor, the in vitro cultured normal human periodontal ligament cells were stimulated to observe the decorin gene expression in the periodontal ligament cells.
METHODS: The periodontal ligament cells were isolated and cultured using trypsin digestion method. The third generation of cells at logarithmic growth phase were preserved in DMEM containing 10% DMSO and 20% FBS frozen cryopreservation fluid, then moved into liquid nitrogen preservation at the second day. Immunohistochemistry was used to identify anti-vimentin and cytokeratin staining. The sixth generation of human periodontal ligament cells were divided into experimental group and control group. The experimental group was cultured in DMEM culture medium containing basic fibroblast growth factor at the concentration of 0.1, 1, 10 μg/L under standard conditions for 24 hours; control group was cultured with DMEM culture medium under standard conditions for 24 hours. The intracellular decorin gene expression was determined using RT-PCR.
RESULTS: By microscopic observation, the cells in the control group had no significant changes, and cells proliferated in the experimental group, before stimulation the cells at the bottom of the bottle became denser than those in sparse regions. Adding basic fibroblast growth factor into periodontal ligament cells, could significantly decrease the mRNA expression of decorin, along with the increase of basic fibroblast growth factor concentration, the inhibition effect gradually weakened and became the strongest in the 0.1 μg/L but the weakest at 10 μg/L.
CONCLUSION: The basic fibroblast growth factor stimulation can promote the proliferation of periodontal ligament cells and inhibit the synthesis of decorin in a dose-dependent manner, 0.1 μg/L induces the strongest inhibitory effect.