Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (11): 1915-1918.doi: 10.3969/j.issn.1673-8225.2010.11.004

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Inhibition of peroxiredoxin Ⅱ on human intervertebral disc cells cultured in vitro

Liu Yu-lin, Zhou Chu-song, Chen Zhong, Luo Ping, Lin Li-jun   

  1. Department of Orthopaedics, Affiliated Zhujiang Hospital of Southern Medical University, Guangzhou  510280, Guangdong Province, China
  • Online:2010-03-12 Published:2010-03-12
  • Contact: Zhou Chu-song, Chief physician, Associate professor, Department of Orthopaedics, Affiliated Zhujiang Hospital of Southern Medical University, Guangzhou 510280, Guangdong Province, China zcsmd@yahoo.com.cn
  • About author:Liu Yu-lin, Studying for master’s degree, Department of Orthopaedics, Affiliated Zhujiang Hospital of Southern Medical University, Guangzhou 510280, Guangdong Province, China liuyulinhp200068888@126.com

Abstract:

BACKGROUND: Intervertebral disc degeneration can reduce nucleus pulposus cells, and peroxiredoxin Ⅱ involved in the regulation of resist oxidation damage, cell division, differentiation, signal transduction and apoptosis. Peroxiredoxin Ⅱ has promotive effect on intervertebral disc degeneration, whereas the mechanism remains poorly understood.    
OBJECTIVE: To observe the effects of peroxiredoxin Ⅱ on human intevertebral disc cells activity and type Ⅱ collagen synthesis in vitro.
METHODS: Human degenerated human lumbar disc cells were cultured in vitro, and assigned into the control and peroxidase Ⅱ groups. Peroxidase Ⅱ with doses of 10, 100 and 1 000 ng/L were added into the peroxidase Ⅱ groups. The cells were identified by immunohistochemical staining, and the cell proliferation was detected using cck-8 kit. Cell supernatant was collected at days 3 and 7 after operation, and the expression of type Ⅱ collagen was measured by double-antibody sandwich enzyme-linked immunosorbent assay.
RESULTS AND CONCLUSION: In vitro cultured human degenerative lumbar intervertebral disc nucleus pulposus cells by adding peroxidase increased with the dose-Ⅱ, the disc nucleus pulposus cells of the volume and type Ⅱ collagen synthesis gradually reduced (P < 0.01). Tips peroxidase Ⅱ on the intervertebral disc nucleus pulposus cells, the number and type Ⅱ collagen synthesis significantly inhibited in a dose-dependent manner. Thus speculated that peroxidase Ⅱ on the nucleus pulposus cells in vitro may lead to disc degeneration as a precipitating factor.

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