Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (27): 5127-5130.doi: 10.3969/j.issn.1673-8225. 2010.27.044

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Protective effect of oyster extract on apoptosis of cerebral neural stem cells induced by hyperthermia

Song Hai-yan1, Song Yong-li2, Yu Qing-mei3, Zhuang Yuan3, Wu Yu-ling4   

  1. 1 Department of Human Anatomy, Xinxiang Medical University, Xinxiang  453003, Henan Province, China; 2 Department of Prevention and Health Care, Second Affiliated Hospital of Shandong Chinese Medical University, Jinan  250001, Shandong Province, China; 3 Department of Human Anatomy, Shandong Medical College, Linyi  276002, Shandong Province, China; 4 Department of Histology and Embryology, Shandong University Medical School, Jinan  250012, Shandong Province, China
  • Online:2010-07-02 Published:2010-07-02
  • Contact: Wu Yu-ling, Professor, Master’s supervisor Department of Histology and Embryology, Shandong University Medical School, Jinan 250012, Shandong Province, China wylp53@sdu.edu.cn
  • About author: Song Hai-yan★, Master, Assistant, Department of Human Anatomy, Xinxiang Medical University, Xinxiang 453003, Henan Province, China zzx@xxmu.edu.cn
  • Supported by:

    the Science and Technology Department of Shandong Province, No. 2005GG4402054*

Abstract:

BACKGROUND: Previous results of our study show that oyster extract has some protective effects on apoptosis of the neuroepithelium in neural tube defects induced by hyperthermia in vivo. It remains unclear whether the extract also protects in vitro cultured neural stem cells.
OBJECTIVE: To investigate the protective effect of oyster extract on apoptosis of cerebral neural stem cells induced by hyperthermia.
METHODS: The cerebral neural stem cells of embryonic mice of 13 days were cultured in vitro. Nestin expression was detected by immunofluorescence method to identify neural stem cells. The neural stem cells of passage 3 were divided randomly into 4 groups: hyperthermia control group and oyster treated I, II, III groups (mass concentration 2.5, 5, 10 g/L oyster extract solution). In addition, culture solution control group (no cells), and culture solution+ oyster extract control group (no cells) were designed. All oyster extract groups and control groups were treated by hyperthermia over 39 °C. The survival rate and the vitality of neural stem cells were detected by trypan blue staining and MTT assay. Western-blotting was employed to explore the expression of p53 in cerebral neural stem cells of each group.
RESULTS AND CONCLUSION: The survival rate and the value of MTT assay in oyster treated groups II and III were significantly greater than hyperthermia control group (P < 0.05), but the expression of p53 in oyster treated groups II and III were weaker than hyperthermia control group. Oyster extract plays an important protective role in the apoptosis of neural stem cells induced by hyperthermia.

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