Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (29): 5437-5440.doi: 10.3969/j.issn. 2095-4344.2012.29. 024

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Effect of quercetin chitosan composite film on superoxide dismutase activity and malondialdehyde content in oral ulcer and wound tissues

Zhao Zhi-yu1, Guo Lan2, Zhao Yu-mei2, Han Shu-ying2   

  1. 1Department of Stomatology, Affiliated Hospital of Hebei United University, Tangshan 063000, Hebei Province, China;
    2Hebei United University, Tangshan 063000, Hebei Province, China
  • Received:2011-12-02 Revised:2012-01-30 Online:2012-07-15 Published:2012-07-15
  • Contact: Han Shu-ying, Professor, Master’s supervisor, Hebei United University, Tangshan 063000, Hebei Province, China shuyinghan59@126.com
  • About author:Zhao Zhi-yu★, Master, Attending physician, Department of Stomatology, Affiliated Hospital of Hebei United University, Tangshan 063000, Hebei Province, China tszzygl@163.com

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Abstract:

BACKGROUND: Quercetin chitosan composite film was prepared by natural absorbable chitosan and quercetin which have anti-inflammation and accelerating effects on wound healing, and natural absorbable chitosan served as film carriers. A primary observation through an animal model on the pharmacological functions of the composite film was done.
OBJECTIVE: To investigate the therapeutic effects of quercetin chitosan composite film on oral ulcer in rats induced by NaOH.
METHODS: A SD rat model of oral ulcer induced by NaOH was established. A total of 80 SD rats were randomly divided into four groups: composite film group was given quercetin chitosan composite film, chitosan film group was given chitosan film, Bingpeng San group was given Bingpeng San and control group received no treatment. Each group was dosed twice a day until the oral ulcer in rats was healed completely. Besides, 10 rats from each group were selected, and a rat model of full-thickness back skin defects on both sides of the spine was established. On day 2 after the model was established, the composite film group was treated with quercetin chitosan solution; the chitosan film group was treated with chitosan solution; the Bingpeng San group was smeared with Bingpeng San solution and the control group underwent no treatments. These groups were dosed twice a day for 3 days.
RESULTS AND CONCLUSION: Compared with the control group, the ulcer area of the composite film group at each time point was smaller (P < 0.01). At days 2 and 4 after administration, the ulcer area of the composite film group was also smaller than that of the Bingpeng San group (P < 0.05). The composite film group was obviously better than the control group at the degrees of ulcer infection (P < 0.01). There was no significant difference between the composite film group and Bingpeng San group. The superoxide dismutase activity in the wound tissue of rats in the composite film group was significantly higher than that in the control group (P < 0.01), while malondialdehyde content in the composite film group was lower than that in the control group (P < 0.01). These findings suggest that quercetin chitosan composite film can promote ulcer wound healing, which may be related with the increase of superoxide dismutase activity and the decrease of malondialdehyde content in the wound tissue.

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