Abstract:

BACKGROUND: It is important to prepare a bone substitute material with good osteoacusis, osteoinductive activity and cell compatibility that is easily molded, which is of significance to repair irregular bone defects and refractory bone fractures.
OBJECTIVE: To prepare demineralized bone matrix/sodium alginate (DBM/ALG) composite material and to screen out the optimal composite ratio, as well as to evaluate its cell compatibility.
METHODS: DBM was prepared from the cortical bone of healthy donors by cleaning, grinding and demineralization. According to the ratio of 5/5, 6/4, 7/3, 8/2, the DBM was compounded with ALG, and the DBM/ALG composite material was prepared through screening the optimal ratio by disperse diameter method. L-929 fibroblasts of mice were cultured in the leaching liquor of DBM/ALG, and the cytotoxicity was tested by MTT method at days 2, 4 and 7.
RESULTS AND CONCLUSION: With the increase of ALG content, the viscosity of DBM/ALG was strengthened and dispersed diameter was reduced gradually. When the ratio of DBM/ALG was 7/3, DBM/ALG was not discrete easily, and dispersed diameter was 3.7 cm in 37 ℃ water. The results of MTT showed that cell proliferation rates were 91.56%, 95.43% and 97.47% at days 2, 4 and 7, respectively; while cytotoxicity was in grade 1.