Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (28): 5145-5148.doi: 10.3969/j.issn. 2095-4344.2012.28. 004

Previous Articles     Next Articles

Effect of intraosseous circulation system damage on the metabolism of adjacent articular cartilage

Liu Jiong1, An Hong2, Zhao Bin1, Li Qing-song1, Chen Yi-xin1   

  1. 1Department of Orthopedics, Fourth People’s Hospital of Guiyang City, Guiyang 550002, Guizhou Province, China;
    2Department of Orthopedics, First Affiliated Hospital of Chongqing Medical University, Chongqing 630014, China
  • Received:2012-01-30 Revised:2012-05-06 Online:2012-07-08 Published:2012-07-08
  • About author:Liu Jiong☆, Doctor, Associate chief physician, Department of Orthopedics, Fourth People’s Hospital of Guiyang City, Guiyang 550002, Guizhou Province, China Guohui0101@ 126.com

PDF

425

Abstract:

BACKGROUND: The implantation of internal fixators into the medullary cavity is a common method for femoral shaft fractures. However, intraosseous circulation system damage can be usually found in this therapeutic process. Pathological changes of adjacent articular cartilage can be found in some patients after treatment, which may be due to the abnormal cartilage metabolism caused by mechanical factors in clinic.
OBJECTIVE: To investigate the effect of intraosseous circulation system damage on the metabolism of adjacent articular cartilage.
METHODS: Totally 24 New Zealand white rabbits were randomly divided into model group, control group and blank group. A rabbit model of circulation system damage was established by injection of bone cement into the medullary cavity of rabbits in the model group. In the control group, the medullary cavity of rabbits was exposed, and then the incision was sutured. In the blank group, the bone marrow cavity was not exposed, but the incision progress was the same as the other two groups. After 4 weeks, all rabbits were killed, and articular cartilage tissues from the femoral knee were extracted.
RESULTS AND CONCLUSION: Hematoxylin-eosin staining showed that in the model group, ulcer and erosion occurred on the surface of cartilage tissues, and the number of chondrocytes was decreased. Besides, disorganized arrangement and vacuolar degeneration were found. In addition, the arrangement of collagen fibers was disorganized and ruptured. In the control and blank groups, the surface of articular cartilage tissues was smooth, and cells and collagen tissues arranged in order. Reverse transcription-PCR detection indicated that mRNA expression of bone morphogenetic protein-2 and transforming growth factor-β1 in the articular cartilage tissues of the model group was lower than that of the control and blank groups. However, there was no significant difference in the mRNA expression of bone morphogenetic protein-2 and transforming growth factor-β1 between the control and blank groups. These results suggest that intraosseous circulation system damage can regulate the mRNA expression of bone morphogenetic protein-2 and transforming growth factor-β1 and influence the metabolism of articular cartilage tissues and collagens.

CLC Number: