Transforming growth factor-beta 3 induces differentiation of adipose-derived mesenchymal stem cells into fibrochondrocytes

doi:10.3969/j.issn.2095-4344.2013.49.017

Abstract

Abstract:

BACKGROUND: The repair of temporomandibular joint disc is still a great clinical challenge. Pluripotent adipose-derived mesenchymal stem cells bring a hope for fibrocartilage tissue repair. Up to now, there are few studies concerning transforming growth factor-β3 to induce differentiation of adipose-derived mesenchymal stem cells into fibrochondrocytes.
OBJECTIVE: To observe the growth and morphology of adipose-derived mesenchymal stem cells under the induction of transforming growth factor-β3.
METHODS: We observed the morphology of fibrochondrocytes differentiated from adipose-derived mesenchymal stem cells under the induction of transforming growth factor-β3. Histological and immunofluorescence staining were employed to detect the expression of extracellular matrix Ⅰ, Ⅱ collagens and proteoglycansthe produced by adipose-derived mesenchymal stem cells. We also evaluated the feasibility of adipose-derived mesenchymal stem cells as seed cells for fibrocartilage tissue engineering.
RESULTS AND CONCLUSION: Under the inverted fluorescence microscope, induced cells showed obvious aggregation growth, polygonal shape, and increased secretion of extracellular matrix. Alcian blue staining results showed that under the induction of transforming growth factor-β3, adipose-derived mesenchymal stem cells exhibited the distinctly deep blue, indicating a large number of glycosaminoglycan was synthesized. Immunostaining results showed that under the induction of transforming growth factor β3, adipose-derived mesenchymal stem cells synthesized extracellular matrix Ⅰ, Ⅱ collagen. These findings suggest that transforming growth factor β3 can induce adipose-derived mesenchymal stem cells to differentiate into fibrouscartilage-like cells, and that adipose-derived mesenchymal stem cells can serve as seed cells for fibrocartilage tissue engineering.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: stem cells, transforming growth factor beta3, adipose tissue, mesenchymal stem cells, fibrocartilage

CLC Number:

R394.2

Lei Sheng-nan, Fan Xing. Transforming growth factor-beta 3 induces differentiation of adipose-derived mesenchymal stem cells into fibrochondrocytes[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(49): 8570-8575.

Figures/Tables 5

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