Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (27): 5050-5053.doi: 10.3969/j.issn.1673-8225.2010.27.025

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Angelica effects on proliferation and differentiation of neural stem cells from neonatal rats with intrauterin hypoxia

Chen Xu-dong1, Zhao Si-min1, Hua Xin-yu1, Yu Hong2   

  1. 1 Luohe Medical College, Luohe  462002, Henan Province, China; 2 Luzhou Medical College, Luzhou  646000, Sichuan Province, China
  • Online:2010-07-02 Published:2010-07-02
  • Contact: Yu Hong, Master, Professor, Master’s supervisor, Luzhou Medical College, Luzhou 646000, Sichuan Province, China
  • About author:Chen Xu-dong★, Master, Lecturer, Luohe Medical College, Luohe 462002, Henan Province, China Lichenyue3@yahoo. com.cn
  • Supported by:

    a Grant of Department of Science and Technology of Sichuan Province, No. [2005]14, 05JY029-103*

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Abstract:

BACKGROUND: Previous studies have found that intrauterin hypoxia could stimulate proliferation of neural stem cells (NSCs) of neonatal rats. The proliferation reached a peak during 6-hours hypoxia; proliferation also expressed in 9 hours, but the ability began to decline, but the neural stem cells showed necrosis or apoptosis when hypoxia up to 12 hours. However, what effect will have on neural stem cells with the time and day extension?
OBJECTIVE: To study the effect of intrauterin hypoxia on the proliferation and differentiation of NSCs from neonatal rats and the protective role of angelica injection on NSCs under hypoxia.
METHODS: Pregnant Sprague Dawley rats were randomly divided into control group, hypoxia group and angelica group. Pregnant rats from angelica group and hypoxia group were placed in the three-gas incubator from the beginning of pregnant 14th days to make the injured brain neonatal rat model. One hour ago, the pregnant rats of the two groups received injections respectively angelica injection and normal saline through the caudal vein of rats. Hypoxia was not performed in the control group. The remaining was identical to hypoxia group. Their brain tissues of neonatal rats were immediately taken out after childbirth. The expression of glial fibrillary acidic protein (GFAP) and neurone specific enolase (NSE) of neonatal rats was studied with immunohistochemistry and the results were analyzed by image processing system.
RESULTS AND CONCLUSION: ①Expression of GFAP-positive cells in the hippocampus of neonatal rats in the hypoxia group was higher than control group. However, expression of NSE-positive cells was less in the hypoxia group than in the control group. ②Expression of GFAP-positive cells in the hippocampus of neonatal rats was less in the angelica group than in the hypoxia group, whereas expression of NSE-positive cells was higher in the angelica group than in the control group. Results have indicated that hypoxia could stimulate the proliferation of NSCs of neonatal rats and differentiation of NSCs into glial cells. Meanwhile, the number of neuron in hippocampus CA3 area was decreased. The ability of proliferation and differentiation of NSCs into glial cells after hypoxia was attenuated by angelica injection. At the same time, angelica injection could relieve neuron decrement. These suggested that angelica injection has a certain protective effect on nervous system of neonatal rats with intrauterine hypoxia.

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