Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (27): 5001-5005.doi: 10.3969/j.issn.1673-8225.2010.27.014

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Inducing human parthenogenetic embryonic stem cells into islet-like clusters

Zhou Jing 1,2, Li Jin 1,2, Lin Ge 1,2, Lu Guang-xiu 1,2   

  1. 1 Institute of Reproductive and Stem Cell Engineering, Central South University, Changsha   410078, Hunan Province, China; 2 National Research and Engineering Center of Human Stem Cells, Changsha    410078, Hunan Province, China
  • Online:2010-07-02 Published:2010-07-02
  • Contact: Lu Guang-xiu, Professor, Institute of Reproductive and Stem Cell Engineering, Central South University, Changsha 410078, Hunan Province, China; National Research and Engineering Center of Human Stem Cells, Changsha 410078, Hunan Province, China lugxdirector@yahoo.com.
  • About author:Zhou Jing☆, Studying for doctorate, Institute of Reproductive and Stem Cell Engineering, Central South University, Changsha 410078, Hunan Province, China; National Research and Engineering Center of Human Stem Cells, Changsha 410078, Hunan Province, China zhoujing12 @gmail.com
  • Supported by:

    the Hi-Tech Research and Development of China (863 program), No. 2006AA02A102*; the National Basic Research Program of China (973 program), No.2005CB522705*; the National Natural Science Foundation of China, No. 30800659*

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Abstract:

BACKGROUND: Following the successful establishment of human parthenogenetic embryonic stem (hpES) cell lines, the further functional study becomes hot spot.
OBJECTIVE: To investigate the potential differentiation ability to islet-like clusters from hpES cell lines.
METHODS: One self-established hpES cell line and one normal hpES cell line were obtained. We adopted a modified 5-stages protocol which based on the development rule of pancreas to induce hpES cells into islet-like clusters.
RESULTS AND CONCLUSION: The terminal differentiated cells gathered into islet-like clusters. The results from immunohistochemistry and RT-PCR showed that the islet-like clusters expressed the islet specific hormones and functional markers. Insulin release test indicated that the clusters had the biochemical function of islet. The islet-like clusters derived from phES cells had the basic characteristics of islet, which would be a reliable material to treat type I diabetes mellitus.

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