Compatibility of human osteoblasts and composite porous bioscaffold

doi:10.3969/j.issn.2095-4344.2013.51.014

Abstract

Abstract:

BACKGROUND: The degradation of a collagen Ⅰ modified porous calcium sulfate scaffold in vivo is unclear, and its degradation product effects on human osteoblasts are rarely reported.
OBJECTIVE: To observe the biocompatibility of human osteoblasts with degradation products of calcium sulfate/collagen membrane composite porous scaffold.
METHODS: Passage 2 human osteoblasts were cultured in the extract of degradation products of calcium sulfate/collagen membrane composite porous scaffold and in Dulbecco’s modified Eagle’s medium containing 10% newborn calf serum. At days 1, 3, 5, 7, cell proliferative curves and total protein were determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method and Coomassie brilliant blue micro-plate method, respectively. And alkaline phosphatase activity was also detected.
RESULTS AND CONCLUSION: The proliferation rate of human osteoblasts in the extract of degradation products of calcium sulfate/collagen membrane composite porous scaffold was slightly higher than that in the Dulbecco’s modified Eagle’s medium containing 10% newborn calf serum, but there was no significant difference (P > 0.05). Alkaline phosphatase activity, total protein synthesis and alkaline phosphatase/total protein were increased with time in the two groups, but there was no significant difference at different time (P > 0.05). These findings indicate that the degradation products of calcium sulfate/collagen membrane composite porous scaffold cannot influence proliferation and growth of human osteoblasts as well as their normal physiological functions, which have good biocompatibility.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: biocompatible materials, tissue engineering, collagen, histocompatibility

CLC Number:

R318

Sun Jiang-wei, Wang Dong, Sun Hai-yu. Compatibility of human osteoblasts and composite porous bioscaffold[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(51): 8869-8874.

Figures/Tables 4

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