Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (19): 3503-3506.doi: 10.3969/j.issn.1673-8225.2011.19.019

Previous Articles     Next Articles

Olfactory ensheathing cell transplantation for treatment of sciatic nerve injury

Yang Shuo, Hou Zhan-jiang, Xia Hao-chen, Fan Chang-dong   

  1. Department of Emergent Trauma Surgery, First Affiliated Hospital of Harbin Medical University, Harbin  150001, Heilongjiang Province, China
  • Received:2010-10-27 Revised:2010-12-06 Online:2011-05-07 Published:2011-05-07
  • Contact: Hou Zhan-jiang, Master, Attending physician, Department of Emergent Trauma Surgery, First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China jbjsam@126.com
  • About author:Yang Shuo★, Master, Attending physician, Department of Emergent Trauma Surgery, First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China jbjsam@126.com

PDF

385

Abstract:

BACKGROUND: Studies have demonstrated that olfactory ensheathing cells (OECs) are conducive to the survival of neurons and promote axonal regeneration.
OBJECTIVE: To study the feasibility and effect of local injection of rat OECs to treat peripheral nerve injury.
METHODS: Rat OECs were isolated and cultured in vitro. 1.0 cm sciatic nerve segment was cut from 40 adult female SD rats undergoing allograft nerve transplantation, and then the rats were randomly divided into two groups. OECs group: local injection of OECs; saline group: local injection of sterile saline. After 3 months, somatosensory evoked potentials (SEP) and motor evoked potentials (MEP) were detected and electrophysiological recovery of nerve was observed under light microscopy and electron microscopy.
RESULTS AND CONCLUSION: The electron microscopy found that in the damage zone, OECs group had more nerve fibers significantly than saline group (P < 0.01); In OECs group, SEP and MEP latency and amplitude was significantly better than that in saline group (P < 0.01). it is shown that local injection of OECs can better restore function after peripheral nerve injury.

CLC Number: