Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (19): 3481-3484.doi: 10.3969/j.issn.1673-8225.2011.19.014

Previous Articles     Next Articles

Effects of mouse placenta-derived mesenchymal stem cells on the natural senescence of the mouse hematopoietic system

Huang Hai-yan1, Liu Cheng-cheng2, Zhu Ai-zhen2, Zhou Yan-hua2, He Dong-mei2, Liu Ge-xiu2, Tan Guang-xiao2   

  1. 1Department of Obstetrics and Gynecology, the First Affiliated Hospital of Jinan University, Guangzhou  510632, Guangdong Province, China
    2Institute of Hematology, Medical College of Jinan University, Guangzhou  510632, Guangdong Province, China
  • Received:2011-01-27 Revised:2011-03-23 Online:2011-05-07 Published:2011-05-07
  • Contact: Liu Ge-xiu, Doctor, Associate investigator, Institute of Hematology, Medical College of Jinan University, Guangzhou 510632, Guangdong Province, China tliugx@jnu.edu.cn
  • About author:Huang Hai-yan, Senior nurse, Department of Obstetrics and Gynecology, the First Affiliated Hospital of Jinan University, Guangzhou 510632, Guangdong Province, China 291568079@qq.com Liu Cheng-cheng★, Studying for master’s degree, Institute of Hematology, Medical College of Jinan University, Guangzhou 510632, Guangdong Province, China liuchengchengf1@163.com Huang Hai-yan and Liu Cheng-cheng contributed equally to this paper.
  • Supported by:

    the National Natural Science Foundation of China, No. 30670902*

PDF

521

Abstract:

BACKGROUND: Hematopoietic system, as an important part of human body, will appear continuously functional recession with age.
OBJECTIVE: To explore the anti-aging role of mouse placenta-derived mesenchymal stem cells (mPMSCs) on the natural senescence of the mouse hematopoietic system.
METHODS: Experimental method: ①Preparation of the mPMSC: the placentas were collected from 6 pregnant 13.5 d Balb/c mice, and digested by 0.1% collagenase IV to obtain the cells, and the cells were plated, passaged, and identified as mPMSCs. ②Intervention of the mPMSCs: 6 month old Balb/c mice (48) were randomly divided into experimental and control groups. Mice of experimental group were injected with mPMSC (5×106 for each one) through tail intravenation, once a month, a total of six times; and mice of control group with physiological saline. Experimental assessment: ①The mPMSCs preparation: the morphology of cells was observed through inverted phase microscope, cell-surface markers by flow cytometry, cell differentiation potentials by inducing osteoblast differentiation and adipocyte differentiation. ②The mPMSCs intervention: on the third month after treatment, peripheral blood (WBC, Hb), bone marrow mononuclear cell count (BMNC), fibroblasts colony culture (CEU-F), hematopoiesis progenitor cell colony culture (CFU-GM, CFU-E, CFU-MK) and exogenous spleen colony-forming units count (CFU-S) were compared between two groups; on the basis of above indexes, the bone marrow histology and the capability of hematopoietic reconstruction of bone marrow cells were added on the sixth month after treatment. 
RESULTS AND CONCLUSION: The general status of mice in the experimental group was better than that in the control group; in the third month, BMNC, CFU-GM and CFU-MK of the experimental group were higher than that of the control group, but no significant difference on the peripheral blood, CFU-F and CFU-E; on the sixth month, all the indexes mentioned above were higher than control group with exception of peripheral blood; during observation period all the indexes had a similar descending-tendency , but the tendency of experimental group was much slower than that of control group with significant difference (P < 0.05). Furthermore, the experimental group had more hematopoietic tissue but less adipose tissue than that of control group; The capacity of hematopoietic reconstruction of bone marrow cells from experimental group was better than that of control group. The results indicated that mPMSCs could delay the natural recession of mouse hematopoietic system.

CLC Number: