Nano-hydroxyapatite/chitosan/alginate for repairing mandibular defects

doi:10.3969/j.issn.2095-4344.2013.51.006

Abstract

Abstract:

BACKGROUND: Studies have confirmed that the nano-hydroxyapatite/chitosan/alginate composite materials have a certain flexibility and strength and possess a bioactivity similar to human bone.
OBJECTIVE: To explore the effect of the nano-hydroxyapatite/chitosan/alginate composite materials on the repair of rabbit mandible defects.
METHODS: Bilateral mandibular defect models of 10 mm × 5 mm × 5 mm were made in 18 healthy New Zealand white rabbits. Then, the rabbits were divided into two groups: experimental group was implanted with nano-hydroxyapatite/chitosan/alginate composite material, and control group was implanted with hydroxyapatite/chitosan composite. At 4, 8 and 12 weeks after implantation, cone-beam CT was applied to observe implant degradation, callus growth and bone connection in the defect area; new bone formation was observed by hematoxylin-eosin staining.
RESULTS AND CONCLUSION: The gray values of the bone density in the experimental group and control group gradually increased with time, and there were remarkably significant differences between the two groups at different time points (P < 0.01). At the same time point, the experimental group was superior to the control group in gross observation, cone-beam CT observation, gray value of CT and histological observation (P < 0.05). At 4-8 weeks after implantation, the implant materials in the two groups were gradually degraded with a blurred junction between the defect and bone tissue, and a small amount of new bone formed tightly integrated to the recipient bone tissue, in which the experimental group was more significant. And during 8 to 12 weeks, the degradation of implanted materials in the two groups was basically complete, and the implant began to merge with the recipient bone tissue, with further generation of new bone tissue and gradual repair of bone defect area, in which, the experimental group was more obvious. Results show that the nano-hydroxyapatite/chitosan/alginate can repair bone defects effectively, and promote the new bone formation.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: biocompatible materials, chitosan, hydroxyapatites, nanocomposites

CLC Number:

R318

Sun Zhang, Meng Cun-fang, Zhang Zhi-quan, Li Shan-chang, Zhou Yang. Nano-hydroxyapatite/chitosan/alginate for repairing mandibular defects[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(51): 8815-8820.

Figures/Tables 4

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