Alendronate improves bone matrix structure of ovariectomized rats

doi:10.3969/j.issn.2095-4344.2013.50.001

Abstract

Abstract:

BACKGROUND: Bisphosphonates that can increase bone density and inhibit bone resorption have been clinically confirmed, but the structure of the bone matrix has been less studied.
OBJECTIVE: To observe the effects of alendronate on bone structure and bone matrix metabolism, and then to investigate the controlling mechanism by which alendronate improves bone mass and increase bone intensity.
METHODS: An ovariectomized rat model was prepared and intervened with alendronate as treatment group. Model and sham-surgery groups were set as controls. Alendronate effects on bone mineral density, bone metabolism, bone biomechanics, and bone structure were observed in bone loss rats using bone imaging, bone tissue pathology and biomechanical test and enzyme-linked immunosorbent assay.
RESULTS AND CONCLUSION: Alendronate intervention could fight against bone loss as compared with model group at weeks 4, 8, and 12 after treatment (P < 0.05). Compared with the model group, the expression of urinary deoxypyridinoline and serum carboxyterminal propeptide of type Ⅰ procollagen was decreased significantly after alendronate intervention (P < 0.05); the maximal load, maximal pressure and modelus on the lumbar vertebrae and femur were increased as well as ratio of urinary pyridinoline/deoxypyridinoline of type Ⅰ procollagen (P < 0.05). These findings suggest that alendronate intervention can inhibit bone loss in rats induced by estrogen deficiency, increase biomechanical properties, improve bone matrix structure, and meanwhile, recover the Ⅰ collagen crosslinking component due to ovariectomy.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: osteoporosis, bone remodeling, ovariectomy, animals

CLC Number:

R318

Wang Xiang, Guo Hai-ling, Zhao Yong-fang, Zhan Hong-sheng, Shi Yin-yu. Alendronate improves bone matrix structure of ovariectomized rats[J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.2013.50.001.

Figures/Tables 6

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