Effects of basic fibroblast growth factor gene transfection on the proliferation of umbilical cord mesenchymal stem cells

doi:10.3969/j.issn.2095-4344.2013.45.010

Abstract

Abstract:

BACKGROUND: At present, exogenous basic fibroblast growth factor gene can be transfected into umbilical cord mesenchymal stem cells via a recombinant adeno-associated virus vector and exhibit sustained expression in transfected cells. This method can regulate cell proliferation and directed differentiation to obtain efficient long-lasting therapeutic effects.
OBJECTIVE: To investigate the effects of basic fibroblast growth factor gene transfection via a recombinant adeno-associated virus vector on the proliferation and cell cycle of human umbilical cord mesenchymal stem cells cultured in vitro.
METHODS: Human umbilical cord mesenchymal stem cells were cultured by the suspension culture in vitro, and were transfected by recombinant adeno-associated virus-mediated basic fibroblast growth factor gene. Cultured cells were divided into three groups: control group, basic fibroblast growth factor group, and recombinant adeno-associated virus group. Reverse transcription-PCR and western blot were used to assess the knockdown efficiency. Cellular proliferation was determined by cell growth curve and Cell Counting Kit-8 assay. The cell cycle was analyzed by flow cytometry.
RESULTS AND CONCLUSION: Compared with the other two groups, the expression of basic fibroblast growth factor mRNA and protein increased significantly, the cell growth speed was also significantly increased, the cell cycle of G0/G1 phase was decreased and cell number in S phase was increased in the basic fibroblast growth factor group after transfection. These findings suggest that the recombinant adeno-associated virus-mediated basic fibroblast growth factor gene can promote the proliferation of umbilical cord mesenchymal stem cells proliferation cultured in vitro, and also can optimize the cell culture.

Key words: stem cells, mesenchymal stem cells, umbilical cord, fibroblast growth factors, genes, adenoviruses, human, cell proliferation

CLC Number:

R394.2

Zheng Ying-juan, Tian Jian-chang . Effects of basic fibroblast growth factor gene transfection on the proliferation of umbilical cord mesenchymal stem cells[J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.2013.45.010.

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