Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (18): 3288-3292.doi: 10.3969/j.issn.1673-8225.2011.18.015

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Epithelial proliferation and apoptosis in heterotopic tracheal transplantation model

Wang Lin-mao1,2, Wu Kai3, Gao Wen2, Chen Chang2, Bao Fang2, Zheng Hui2   

  1. 1Medical College of Soochow University, Suzhou  215000, Jiangsu Province, China
    2Department of Thoracic Surgery, Affiliated Shanghai Pulmonary Hospital, Medical College of Tongji University, Shanghai   200433, China
    3Department of General Surgery, Yiyuan Country People’s Hospital, Zibo   256100, Shandong Province, China
  • Received:2010-10-13 Revised:2010-11-06 Online:2011-04-30 Published:2011-04-30
  • Contact: Gao Wen, Professor, Chief physician, Department of Thoracic Surgery, Affiliated Shanghai Pulmonary Hospital, Medical College of Tongji University, Shanghai 200433, China gaowen5921@163.com
  • About author:Wang Lin-mao★, Studying for master’s degree, Medical College of Soochow University, Suzhou 215000, Jiangsu Province, China; Department of Thoracic Surgery, Affiliated Shanghai Pulmonary Hospital, Medical College of Tongji University, Shanghai 200433, China wanglinmao12345@sina.com Wu Kai, Master, Department of General Surgery, Yiyuan Country People’s Hospital, Zibo 256100, Shandong Province, China Wang Lin-mao and Wu Kai contributed equally to this paper

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Abstract:

BACKGROUND: The process of epithelial cells in heterotopic trachea transplantation remains unclear. The studies demonstrated that epithelial proliferation is accompanied by irreversible cell death, and airway epithelial regeneration and death occurred before airway occlusion transplantation.
OBJECTIVE: To observe epithelial proliferation and apoptosis in the process of heterotopic mouse trachea transplantation.
METHODS: The allogeneic BALB/c mouse trachea was implanted subcutaneously in the nape of BALB/c mouse. Ciclosporin A (CsA) was not injected after transplantation. The allogeneic BALB/c mouse trachea was implanted subcutaneously in the nape of C57BL/6 mouse. One group was not injected with CsA, the other group was the whole course injected with CsA in abdominal cavity. At 3, 7, 14, 21, 30 days after transplantation, the integrity of airway epithelium, submucosal infiltration of inflammatory cells and fibrous tissue proliferation were evaluated by hematoxylin-eosin staining. BrdU labelling index and TUNEL positive cells in trachea transplantation were determined.
RESULTS AND CONCLUSION: At 3 days after receptor BALB/c mouse transplantation, airway epithelial cells suffered from minor injury and loss. At 7-21 days after transplantation, airway epithelial cells recovered normal gradually. Airway epithelium approached to normal epithelium at 30 days after transplantation. The extent of BrdU labelling index was 3%- 5%. The number of TUNEL positive cells was basically unchanged. At 3 days after receptor C57BL/6 mouse transplantation, airway epithelial cells suffered from minor injury. The shedding and necrosis were existed in airway epithelium, at 7-21 days after transplantation; fibrous proliferation was appeared and became more serious gradually. At 30 days after transplantation, epithelial cells completely disappeared, lumens were filled by fibrous tissue, closed to occlusion. The peak of BrdU labelling index was at 14 days after transplantation. The group of not injected with CsA was higher than group of injected with CsA in BrdU labelling indexes (P=0.000). At 21 and 30 days after transplantation, the BrdU labelling index was significantly reduced. The peak of UNEL positive cells in group of not injected with CsA was at 21 days after transplantation, the peak of UNEL positive cells group of injected with CsA was at 14 days after transplantation. It is indicated that epithelial proliferation in heterotopic trachea transplantation was accompanied by irreversible cell death. CsA could reduce the incidence of bronchiolitis obliterans, but did not prevent its occurrence.

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