Natural hirudin hydrogel improves the survival rate of random pattern flaps

doi:10.3969/j.issn.2095-4344.2013.53.002

Abstract

Abstract:

BACKGROUND: A random pattern flap is prone to resulting in blood stasis and even ischemic necrosis. Clinical and animal experiments have proved that hirudin can improve the survival rate of the pattern flap.

OBJECTIVE: To discuss the effects of natural hirudin hydrogel on the survival of rat random pattern flaps.

METHODS: Natural hirudin hydrogel was prepared for percutaneous permeation experiment using modified Franz diffusion cell. Markwardt method was used to detect hirudin activity. Random pattern flaps from the back of Wistar rats, 9 cm × 3 cm, were used for flap preparation. The flaps were randomized into three groups, normal saline group, hirudin group and blank gel matrix group. Postoperative gross, histopathological, and morphological observations were conducted, and the survival rate of the flaps was calculated at day 7.

RESULTS AND CONCLUSION: In vitro experiments: The active ingredient of the natural hirudin hydrogel passed through the skin, but there was no active ingredient in the blank gel group. In vivo experiments: After 7 days, the length of necrotic flaps in the hirudin group was significantly longer than that in the normal saline and blank gel matrix groups (P < 0.05). After 3 days, the phenomenon of red blood cell stasis in dermal capillaries and venules was significantly reduced in the hirudin group as compared with the normal saline and blank gel matrix groups; after 7 days, subcutaneous collagen and granulation tissue hyperplasia was more obvious in the hirudin group than in the normal saline and blank gel groups; after 7 days, the survival rate of flaps in the hirudin group was significantly higher than that in the normal saline and blank gel matrix groups (P < 0.05). Natural hirudin permeate the intact skin tissue, and local application of natural hirudin hydrogel can improve blood stasis after flap transplantation and be conducive to the formation of new blood vessels.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

全文链接：

Key words: hirudins, gels, osmosis, surgical flaps, rats, Wistar

CLC Number:

R318

Wei Shu-yi, Yin Guo-qian, Han Zhi-qiang, Pan Xin-yuan, Lin Bo-jie. Natural hirudin hydrogel improves the survival rate of random pattern flaps[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(53): 9107-9112.

Figures/Tables 3

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