%A Jiang Lixia, Yuan Ruijuan %T Osteopractic total flavone promotes periosteal cell proliferation and treats the nonunion in rabbits %0 Journal Article %D 2019 %J Chinese Journal of Tissue Engineering Research %R 10.3969/j.issn.2095-4344.1239 %P 2953-2958 %V 23 %N 19 %U {https://www.cjter.com/CN/abstract/article_14629.shtml} %8 2019-07-08 %X

BACKGROUND: Osteopractic total flavone is a traditional Chinese medicine, and has the effects of preventing osteoporosis and regulating blood lipids. The effect of osteopractic total flavone on periosteal cells is little known.
OBJECTIVE: To investigate the effect of osteopractic total flavone on the proliferation of rabbit periosteal cells, and to further study its therapeutic effect on nonunion in rabbits.
METHODS: (1) The third generation of rabbit periosteal cells was cultured with 10-5, 10-6 and 10-7 g/L osteopractic total flavone and 10-8 g/L estradiol, separately. The cell proliferation was detected at 1, 2, 4 and 6 days. (2) The third generation of rabbit periosteal cells was cultured by 10-5 g/L osteopractic total flavone, 10-8 g/L estradiol and purified water. The alkaline phosphatase activity was detected at 1, 5, 9 and 13 days. The count of calcium nodules was measured by Von Kossa assay at 5, 10, 15 and 20 days. (3) The third generation of rabbit periosteal cells was cultured by 10-5 g/L osteopractic total flavone, 10-5 g/L osteopractic total flavone +1 μmol/L ICI182780 (estrogen receptor antagonist) and PBS, separately. The cell proliferation was detected at 1, 2, 4 and 6 days. (4) The rabbit model of nonunion was established, and 10-5 g/L osteopractic total flavone and 1×103 periosteal cells were injected into the nonunion area to observe the effects on the nonunion.
RESULTS AND CONCLUSION: (1) The absorbance value of periosteal cells was increased with time, especially in the 10-5 g/L osteopractic total flavone and estradiol groups, and peaked on day 6. (2) The alkaline phosphatase activity in the 10-5 g/L osteopractic total flavone and estradiol groups was higher than that in the control group (P < 0.05), and there was significant difference between 10-5 g/L osteopractic total flavone and estradiol groups (P > 0.05). (3) The cell proliferation induced by osteopractic total flavone was completely inhibited by 10-8 mol/L estrogen receptor antagonist ICI182780. There was no significant difference in the absorbance value between antagonist and control groups (P > 0.05). (4) The combination of osteopractic total flavone and periosteal cells could promote bone healing. (5) These results imply that osteopractic total flavone can promote osteoblast proliferation and differentiation, which is related to estrogen receptors. Osteopractic total flavone combined with periosteal cells can cure nonunion in vivo.

中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程