%A Wang Lei, Liu Zi-quan, Hou Yi-ling, Ge Yao-jun %T Hydrogen-rich water inhibits mitochondrial oxidative stress and inflammation in the skeletal muscle after eccentric exercise  %0 Journal Article %D 2015 %J Chinese Journal of Tissue Engineering Research %R 10.3969/j.issn.2095-4344.2015.29.018 %P 4682-4687 %V 19 %N 29 %U {https://www.cjter.com/CN/abstract/article_10383.shtml} %8 2015-07-09 %X

BACKGROUND: It is unclear whether hydrogen-rich water can be used to protect skeletal muscle injury induced by eccentric exercise, as well as the relative mechanism.
OBJECTIVE: To observe the effect of hydrogen-rich water on the mitochondrial oxidative stress and inflammation in rat skeletal muscle after eccentric exercise, and to investigate the relative signaling pathway of hydrogen-rich water.
METHODS: Forty Sprague Dawley rats were randomly divided into four groups: control group, eccentric exercise group, eccentric exercise+saline group, and eccentric exercise+hydrogen-rich water group. Rats in three eccentric exercise groups were exercised on a motor-driven rodent treadmill at a speed of 16-18 m/min and a slope of -16° for 90 minutes per day. Rats in the eccentric exercise+hydrogen-rich water group were subjected to intraperitoneal injection of hydrogen-rich water (10 mL/kg) immediately after exercise; and rats in the eccentric exercise+saline group were administrated with normal saline after exercise. All the interventions lasted for 5 days.
RESULTS AND CONCLUSION: Hydrogen-rich water intervention after eccentric exercise could markedly enhance the mitochondrial Sirtuin-3 expression, improve the mitochondrial membrane potential and activity of manganese superoxide dismutase, down-regulate the mitochondrial reactive oxygen species generation and 
mitochondrial DNA oxidative damage, thus inhibiting inflammatory cytokines expression, such as NLRP3 and interleukin-1β. The results indicated that hydrogen-rich saline could directly scavenge reactive oxygen species. In addition, hydrogen-rich water could improve mitochondrial energy metabolism and antioxidant capacity through up-regulation of Sirtuin-3, which in turn inhibits eccentric exercise-induced mitochondrial oxidative stress and secondary inflammation in the skeletal muscle. 

中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程