Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (17): 3078-3081.doi: 10.3969/j.issn.1673-8225.2012.17.010

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Formation capability of bacterial biofilm on titanium plate versus necrotic bone

Zhang Zhi-hong, Sun Xiao-tang, Feng An-ping, Wang Wan-ming   

  1. Second Department of Orthopedics and Traumatology, Fuzhou General Hospital of Nanjing Military Area Command of Chinese PLA, Fuzhou 350025, Fujian Province, China
  • Received:2012-01-04 Revised:2012-03-02 Online:2012-04-22 Published:2012-04-22
  • Contact: Sun Xiao-tang, Doctor, Attending physician, Second Department of Orthopedics and Traumatology, Fuzhou General Hospital of Nanjing Military Area Command of Chinese PLA, Fuzhou 350025, Fujian Province, China xiaotangsun@hotmail.com
  • About author:Zhang Zhi-hong, Associate chief physician, Second Department of Orthopedics and Traumatology, Fuzhou General Hospital of Nanjing Military Area Command of Chinese PLA, Fuzhou 350025, Fujian Province, China zhihongzhang85@yahoo.com.cn
  • Supported by:

     the Natural Science Foundation of Fujian Province, No. 2009J05082*

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Abstract:

BACKGROUND: Bacteria attachment and biofilm formation at the surface of metal implants and sequestrum are major reasons for chronic infection of musculoskeletal system.
OBJECTIVE: To characterize and compare the formation capability of bacterial biofilm on titanium plate and necrotic bone.
METHODS: The model of bacterial biofilm was developed with modified stroma culture approach. Ten titanium plate cylinders and ten pieces of necrotic bone were randomly matched. Each pair was placed in one culture flask and immersed in bacteria solution. The biofilm of each group was stained with fluorochrome, observed and photographed using confocal laser scanning microscope.
RESULTS AND CONCLUSION: Compared with the necrotic bone, the thickness of bacterial biofilm on the titanium plate was less
(P < 0.05) and the percentage of live bacteria in the medium layer and the bottom layer was higher (P < 0.05). Results indicate that the bacterial biofilm tends to more easily develop on the surface of necrotic bone than on the surface of titanium plate.

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