Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (47): 8795-8799.doi: 10.3969/j.issn.1673-8225.2010.47.014

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Proliferation and differentiation of bone marrow mesenchymal stem cells cultured on hydroxyapatite/collagen composite scaffolds

Song Qin1, Yan Jun1, Guo Xiao-qiang1, Yao Qian1, Yu Xiao-bing○2,3   

  1. 1 College of Biotechnology Industry, Chengdu University, Chengdu   610106, Sichuan Province, China; 2 Koken Bioscience Institute, Tokyo   1150051, Japan; 3 Laboratory of Tissue Regeneration, Xinxiang Medical College, Xinxiang   453003, Henan Province, China
  • Online:2010-11-19 Published:2010-11-19
  • Contact: Yu Xiao-bing, Professor, Doctor, Koken Bioscience Institute, Tokyo 1150051, Japan; Laboratory of Tissue Regeneration, Xinxiang Medical College, Xinxiang 453003, Henan Province, China
  • About author:Song Qin☆, Doctor, Lecturer, College of Biotechnology Industry, Chengdu University, Chengdu 610106, Sichuan Province, China sq82802@hotmail.com

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Abstract:

BACKGROUND: Presently collagen as cell three-dimensional scaffold in vitro, has been mainly used in tissue engineering. But collagen scaffolds has low mechanical strength, and weak fatigue resistance. Therefore hydroxyapatite/collagen composite scaffolds are prepared to explore the biocompatibility and bioactivity of the composite scaffold.
OBJECTIVE: To investigate the proliferation and differentiation of bone marrow mesenchymal stem cells (MSCs) cultured on hydroxyapatite/collagen composite scaffolds.
METHODS: MSCs were isolated from SD rats using adherence method and inoculated into the dish, collagen scaffold and hydroxyapatite/collagen composite scaffold respectively. The cellular growth under three conditions was observed, the cellular proliferation was assessed using MTT assay. The differentiation of MSCs was characterized by alkaline phosphatase activity and the secretion of collagen at 14 and 21 days after the culture.
RESULTS AND CONCLUSION: The MSCs well spread on the hydroxyapatite/collagen composite scaffolds, and showed a higher proliferation rate than that cultured on the collagen scaffold and dish. The quantitative examination of alkaline phosphatase activity and secretion of collagen indicated that the cells cultured on the hydroxyapatite/collagen composite scaffolds expressed a higher level than that on the collagen scaffold and dish. The hydroxyapatite/collagen composite scaffold can promote the differentiation and proliferation of MSCs, and induce MSCs differentiate into osteoblasts.

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