Effects of polylactic acid-glycolic acid copolymer/lysine-grafted graphene oxide nanoparticle composite scaffolds on osteogenic differentiation of MC3T3 cells

doi:10.12307/2025.259

Abstract

Abstract: BACKGROUND: How to effectively promote bone regeneration and bone reconstruction after bone injury has always been a key issue in clinical bone repair research. The use of biological and degradable materials loaded with bioactive factors to treat bone defects has excellent application prospects in bone repair.
OBJECTIVE: To investigate the effect of polylactic acid-glycolic acid copolymer (PLGA) composite scaffold modified by lysine-grafted graphene oxide nanoparticles (LGA-g-GO) on osteogenic differentiation and new bone formation.
METHODS: PLGA was dissolved in dichloromethane and PLGA scaffold was prepared by solvent evaporation method. PLGA/GO composite scaffolds were prepared by dispersing graphene oxide uniformly in PLGA solution. LGA-g-GO nanoparticles were prepared by chemical grafting method, and the PLGA/LGA-g-GO composite scaffolds were constructed by blending LGA-g-GO nanoparticles at different mass ratios (1%, 2%, and 3%) with PLGA. The micromorphology,
hydrophilicity, and protein adsorption capacity of scaffolds of five groups were characterized. MC3T3 cells were inoculated on the surface of scaffolds of five groups to detect cell proliferation and osteogenic differentiation.
RESULTS AND CONCLUSION: (1) The surface of PLGA scaffolds was smooth and flat under scanning electron microscope, while the surface of the other four scaffolds was rough. The surface roughness of the composite scaffolds increased with the increase of the addition of LGA-g-GO nanoparticles. The water contact angle of PLGA/LGA-g-GO (3%) composite scaffolds was lower than that of the other four groups (P < 0.05). The protein adsorption capacity of PLGA/LGA-g-GO (1%, 2%, and 3%) composite scaffolds was stronger than PLGA and PLGA/GO scaffolds (P < 0.05). (2) CCK-8 assay showed that PLGA/LGA-g-GO (2%, 3%) composite scaffold could promote the proliferation of MC3T3 cells. Alkaline phosphatase staining and alizarin red staining showed that the cell alkaline phosphatase activity in PLGA/LGA-g-GO (2%, 3%) group was higher than that in the other three groups (P < 0.05). The calcium deposition in the PLGA/GO and PLGA/LGA-g-GO (1%, 2%, and 3%) groups was higher than that in the PLGA group (P < 0.05). (3) In summary, PLGA/LGA-g-GO composite scaffold can promote the proliferation and osteogenic differentiation of osteoblasts, and is conducive to bone regeneration and bone reconstruction after bone injury.

Key words: polylactic acid-glycolic acid copolymer, lysine, graphene oxide, nanoparticle, composite scaffold, bone regeneration, biological tissue engineering

CLC Number:

Yu Shuangqi, Ding Fan, Wan Song, Chen Wei, Zhang Xuejun, Chen Dong, Li Qiang, Lin Zuoli. Effects of polylactic acid-glycolic acid copolymer/lysine-grafted graphene oxide nanoparticle composite scaffolds on osteogenic differentiation of MC3T3 cells[J]. Chinese Journal of Tissue Engineering Research, 2025, 29(4): 707-712.

Figures/Tables 6

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