Abstract:

BACKGROUND: Several studies have shown that many features of nanomaterials can influence nanomaterials toxicity obviously, such as their size, shape and crystallinity.
OBJECTIVE: To explore the effect of material shapes on the biological behaviors of MG63 cells and to analyze the correlations between their physical-chemical properties and biological effect by using three kinds of calcium phosphate nanoparticles with different shapes.
METHODS: Spherical, fusiform and rod-like calcium phosphate nanoparticles were synthesized by hydro-thermal method and chemical precipitation method and filmed on the glasses. The size, shape, roughness and crystallinity of calcium phosphate nanoparticles and their films were characterized by transmission electron microscope, field emission scanning electron microscopy, atomic force microscopy, dynamic light scattering and X-ray diffraction. MG63 cells were seeded and cultured on films. Effects of calcium phosphate on the biological behaviors of MG63 were detected, including adhesion, proliferation, differentiation, and apoptosis.
RESULTS AND CONCLUSION: The results showed that the shape and crystallinity of calcium phosphate influenced MG63 cells proliferation obviously. Calcium phosphate nanoparticles with high aspect ratio and high crystallinity could promote cells proliferation, and conversely, calcium phosphate nanoparticles with low aspect ration and low crystallinity could inhibit cells proliferation. MG63 cells were sensitive to the roughness of calcium phosphate films, and cell adhesion ability was decreased with the increase of the roughness of calcium phosphate films. These findings suggest that the three kinds of calcium phosphate can promote cells migration effectively, but there are no obvious influences on cells differentiation and apoptosis.