Isolation, culture and identification of New Zealand white rabbit talar chondrocytes

doi:10.3969/j.issn.2095-4344.2017.28.008

Abstract

Abstract:

BACKGROUND: Based on the previous studies, the New Zealand rabbit talar chondrocyts were isolated, cultured and identified in vitro.
OBJECTIVE: To explore the isolation, culture and identification of New Zealand rabbit talar chondrocyts in vitro.
METHODS: The chondrocyts were isolated from the talar cartilage of New Zealand white rabbits by type II collagen enzyme digestion, and then cultured in vitro. The cells were identified by inverted phase contrast microscope, toluidine blue staining and collagen type II immunohistochemical staining.
RESULTS AND CONCLUSION: Under the inverted phase contrast microscope, most of passaged chondrocytes presented with polygonal or triangle shape and had round or oval nuclei. Toluidine blue staining showed the hyacinthine chondrocytes and blue cellular matrix. Collagen type II immunohistochemical staining showed that the chondrocytes appeared with brown granules in the cytoplasm and membrane. To conclude, a system that can isolate, culture and identify talar chondrocytes from New Zealand rabbits is successfully established. Talar chondrocytes at passages 1-3 grow well and have stable biological properties.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Talus, Chondrocytes, Collagen Type II, Tissue Engineering

CLC Number:

R318

Wang Yong-ping, Zhu Zhao-jin, Xu Xiang-yang. Isolation, culture and identification of New Zealand white rabbit talar chondrocytes[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(28): 4475-4480.

Figures/Tables 1

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