A tissue-engineered bone constructed with bone marrow mesenchymal stem cells and calcium sulfate artificial bone: its osteoinductive effects in spinal fusion

doi:10.3969/j.issn.2095-4344.2016.43.002

Abstract

Abstract:

BACKGROUND: Biodegrable calcium sulfate artificial bone has a good biocompatibility, so it is used as a bone graft substitute in the treatment of spinal fusion.
OBJECTIVE: To investigate the osteoinductive effects of the tissue-engineered bone made of bone marrow mesenchymal stem cells and calcium sulfate artificial bone in spinal fusion.
METHODS: Bone marrow mesenchymal stem cells were cultured in vitro, and then combined with the calcium sulfate artificial bone. The composite material was observed under electron microscope. Totally 67 patients undergoing spinal fusion were enrolled, who were divided into control group (n=35) and observation group (n=32), receiving autologous iliac bone graft and autologous bone marrow mesenchymal stem cells combined with calcium sulfate transplantation, respectively. Subsequently, spinal fusion Lenke classification and low back outcome score were conducted.
RESULTS AND CONCLUSION: Under electron microscope, the visible calcium sulfate artificial bone presented a good porous structure, on which bone marrow mesenchymal stem cells grew and adhered well. Slightly but insignificantly better outcomes in the spinal fusion through the use of the Lenke classification system were obtained in the observation group than the control group after surgery (P > 0.05). Besides, scores on low back outcomes in both two groups were significantly higher than baseline data (P < 0.05). These results suggest that the tissue-engineered bone made of calcium sulfate artificial bone as the scaffold and bone marrow mesenchymal stem cells as seed cells can exert a good osteoinduction in spinal fusion, and obtain ideal effects.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Stem Cells, Tissue Engineering, Spine

CLC Number:

R318

Ying Lv-fang, Jia Xu-feng, Cheng Yin, Feng Da-xiong.

A tissue-engineered bone constructed with bone marrow mesenchymal stem cells and calcium sulfate artificial bone: its osteoinductive effects in spinal fusion

[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(43): 6396-6402.

Figures/Tables 7

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