Human epidermal stem cells combined with acellular dermal scaffold in nude mice for skin transplantation

doi:10.3969/j.issn.2095-4344.2016.25.019

Abstract

Abstract:

BACKGROUND: Many factors can lead to a large area of skin defects, and tissue-engineered artificial skill transplantation composed by seeding cells and scaffold materials can be used for skin defect repair.

OBJECTIVE: To construct the skin implantation scaffold based on human epidermal stem cells combined with acellular dermal matrix in nude mice.

METHODS: Human epidermal stem cells from children’s foreskin were isolated and cultured, and the skin of nude mice was obtained to prepare acellular dermal matrix scaffold. Then, the human epidermal stem cells were cultured on the acellular dermal matrix scaffold to construct artificial skin. Ten Sprague-Dawley rats were equivalently randomly divided into two groups: rats treated by human epidermal stem cells combined with acellular dermal matrix scaffold as combined group, and those treated by simple acellular dermal scaffold as acellular dermal scaffold group after skin defect models were prepared.

RESULTS AND CONCLUSION: The artificial skin was milky white, soft and elastic, not easy to break, and has good flexibility, and good plasticity, and could be processed into different shapes. No obvious exudation appeared in the rat wound of two groups after repair. At 2 weeks after modeling, the transplanted skin showed good growth and skin wound healed gradually in the combined group. In contrast, scar healing and two animals with transplant failure occurred in the acellular dermal scaffold group. These results show that human epidermal stem cells combined with acellular dermal materials in nude mice can construct the tissue-engineered skin, which exerts good repair effects for skin defects in animals.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Epidermis, Stents, Tissue Engineering

CLC Number:

R318

Zhao Yue-hua, Zhang Yong-hong, Lin Kai. Human epidermal stem cells combined with acellular dermal scaffold in nude mice for skin transplantation[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(25): 3771-3777.

Figures/Tables 5

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