Biocompatibility of zein scaffold and its application in periodontal defect repair

doi:10.3969/j.issn.2095-4344.2016.25.012

Abstract

Abstract:

BACKGROUND: Zein has excellent solubility, heat resistance and biodegradability, but its biocompatibility and effect on periodontal defects repair are under discussion.

OBJECTIVE: To analyze biocompatibility of zein and its effect on periodontal defect repair.

METHODS: Zein scaffold was prepared by solvent casting/particulate leaching. In vitro test: Human periodontal ligament cells were co-cultured with zein scaffold for 18 days, and cell growth was observed by scanning electron microscope. In vivo test: Eight Beagle dogs were enrolled to establish periodontal defect models, which were randomly assigned to receive zein scaffold implantation as experimental group, or interrupted suture as control group. Afterwards, the defect region was observed by scanning electron microscope at 3 months.

RESULTS AND CONCLUSION: In vitro results: Human periodontal ligament cells adhered well and tightly on the scaffold with a fusiform, and could grow around pores. In vivo results: In the experimental group the scaffold dissolved completely, bone trabecular arranged regularly, and mature tissues appeared, to be integrated with the surrounding tissues; in the control group, the defect region almost healed, but there were irregular fibers and obvious lacunae. Moreover, compared with the control group, the height of new alveolar bone and bone defect, as well as the length of junctional epithelium were significantly decreased, and new cementum was significantly increased in the experimental group (P < 0.05). To conclude, zein scaffold has good biocompatibility and can promote periodontal defect repair.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Zein, Materials Testing, Periodontitis, Tissue Engineering

CLC Number:

R318

Li Min, Wang Yao, Wang Shuang-shuang, Li Bei, Yang Lin. Biocompatibility of zein scaffold and its application in periodontal defect repair[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(25): 3726-3731.

Figures/Tables 3

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