Effect of cholestatic serum on differentiation of bone marrow mesenchymal stem cells into hepatocytes

doi:10.3969/j.issn.2095-4344.2016.06.002

Chinese Journal of Tissue Engineering Research ›› 2016, Vol. 20 ›› Issue (6): 771-776.doi: 10.3969/j.issn.2095-4344.2016.06.002

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Effect of cholestatic serum on differentiation of bone marrow mesenchymal stem cells into hepatocytes

Li Wei¹, Wang Lin², Zeng Jie¹, Chen Fang¹

¹Department of Emergency, Sichuan Academy of Medical Sciences & Sichuan Provincial People’s Hospital, Chengdu 610000, Sichuan Province, China; ²Institute of Experimental Animals, Sichuan Academy of Medical Sciences, Chengdu 610031, Sichuan Province, China

Received:2016-01-07 Online:2016-02-05 Published:2016-02-05
Contact: Chen Fang, Master, Chief physician, Department of Emergency, Sichuan Academy of Medical Sciences & Sichuan Provincial People’s Hospital, Chengdu 610000, Sichuan Province, China
About author:Li Wei, Studying for master’s degree, Associate chief physician, Department of Emergency, Sichuan Academy of Medical Sciences & Sichuan Provincial People’s Hospital, Chengdu 610000, Sichuan Province, China
Supported by:
a grant from Sichuan Provincial Health Department, No. 090498

Abstract

Abstract:

BACKGROUND: Under certain conditions, bone marrow mesenchymal stem cells can be differentiated into hepatocytes, which are an important source of liver cells. Moreover, multiple factors can be involved in this induced differentiation process.

OBJECTIVE: To investigate the inducible effect of cholestatic serum on the differentiation of bone marrow mesenchymal stem cells into hepatocytes.

METHODS: Cholestatic animal model was prepared in rats to extract cholestatic serum. Bone marrow mesenchymal stem cells isolated from rats were divided into three groups and cultured in serum-free hepatocyte medium, serum-free hepatocyte medium plus cholestatic serum, serum-free hepatocyte medium plus normal serum, respectively.

RESULTS AND CONCLUSION: The positive expression of alpha fetoprotein and keratin 18 and mass concentration of albumin were significantly higher in the serum-free hepatocyte medium plus cholestatic serum group than the other two groups (P < 0.05). These findings indicate that cholestatic serum has a certain inducible role in the differentiation of bone marrow mesenchymal stem cells into hepatocytes.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Li Wei, Wang Lin, Zeng Jie, Chen Fang. Effect of cholestatic serum on differentiation of bone marrow mesenchymal stem cells into hepatocytes[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(6): 771-776.

Figures/Tables 4

References

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Effect of cholestatic serum on differentiation of bone marrow mesenchymal stem cells into hepatocytes

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