Human bone marrow mesenchymal stem cells promote epithelial mesenchymal  transition in lung cancer cells

doi:10.3969/j.issn.2095-4344.2016.07.011

Chinese Journal of Tissue Engineering Research ›› 2016, Vol. 20 ›› Issue (7): 993-999.doi: 10.3969/j.issn.2095-4344.2016.07.011

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Human bone marrow mesenchymal stem cells promote epithelial mesenchymal transition in lung cancer cells

Wu Jia-bin¹, Wang Tao², Yang Wei-lin¹, Wang Jun-jie², Xiao Jie-fei¹, Wang Ru-chen¹, Chen Zhen-guang¹

¹the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510000, Guangdong Province, China; ²Center for Stem Cell Biology and Tissue Engineering, Sun Yat-sen University, Guangzhou 510000, Guangdong Province, China

Received:2015-01-12 Online:2016-02-12 Published:2016-02-12
Contact: Chen Zhen-guang, M.D., Professor, Chief physician, Master’s supervisor, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510000, Guangdong Province, China
About author:Wu Jia-bin, Studying for master’s degree, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510000, Guangdong Province, China
Supported by:
the National Natural Science Foundation of China, No. 81101774; the China Postdoctoral Science Foundation, No. 2014M552218

Abstract

Abstract:

BACKGROUND: The complex relationship between bone marrow mesenchymal stem cells and cancers severely limit the clinical application of mesenchymal stem cells. So it is urgent to study the role of mesenchymal stem cells in tumor growth and metastasis.
OBJECTIVE: To explore the effect of human bone marrow mesenchymal stem cells on epithelial mesenchymal transition in non-small cell lung cancer A549 and PAa cells.
METHODS: The A549 and PAa cells were cultured with mesenchymal stem cell supernatant (mesenchymal stem cell conditioned medium, MSCs-CM). The cellular morphology was observed under a microscope. The mRNA and protein expression of E-cadherin, N-cadherin, Vimentin, Slug, Snail, and Twist were determined by RT-PCR and western blot. Transwell and wound healing assay were used to detect the change of migration and metastatic ability.
RESULTS AND CONCLUSION: Compared with the control group, the cellular morphology of experimental group showed mesenchymal-like changes. In response to MSCs-CM, there was decreased E-cadherin but increased N-cadherin, Vimentin and Slug, Snail, Twist at mRNA and protein levels compared with the control group (P < 0.05). The migration and metastatic abilities of the experimental group were also increased. So, human bone marrow mesenchymal stem cells can promote epithelial mesenchymal transition in A549 and PAa cells, and enhance the migration and metastatic abilities of A549 and PAa cells.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Wu Jia-bin, Wang Tao, Yang Wei-lin, Wang Jun-jie, Xiao Jie-fei, Wang Ru-chen, Chen Zhen-guang . Human bone marrow mesenchymal stem cells promote epithelial mesenchymal transition in lung cancer cells[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(7): 993-999.

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Human bone marrow mesenchymal stem cells promote epithelial mesenchymal transition in lung cancer cells

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