Histological observation on new bone induced by platelet-rich fibrin

doi:10.3969/j.issn.2095-4344.2016.07.003

Chinese Journal of Tissue Engineering Research ›› 2016, Vol. 20 ›› Issue (7): 933-939.doi: 10.3969/j.issn.2095-4344.2016.07.003

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Histological observation on new bone induced by platelet-rich fibrin

Fu Dong-mei^{1, 2}, Xiao Qiong³, Yang Qin-qiu⁴, Dong Lu⁵, Chen Hong-liang², Sun Yong²

¹School of Stomatology, Southwest Medical University, Luzhou 646000, Sichuan Province, China; ²Chengdu Military Authorities Hospital of PLA, Oral Implant and Prosthodontics Center of Chengdo Military Region, Chengdu 610041, Sichuan Province, China; ³Jiulongpo Dental Hospital of Chongqing Bybo Dental Hospital Administrative Limited Company, Chongqing 400000, China; ⁴Department of Stomatology, the First People’s Hospital of Suining, Suining 629000, Sichuan Province, China; ⁵Department of Stomatology, the First People's Hospital of Liangshan Yi Autonomous Prefecture, Xichang 615000, Sichuan Province, China

Received:2015-11-30 Online:2016-02-12 Published:2016-02-12
Contact: Sun Yong, Chief physician, Master’s supervisor, Professor, Chengdu Military Authorities Hospital of PLA, Oral Implant and Prosthodontics Center of Chengdu Military Region, Chengdu 610041, Sichuan Province, China
About author:Fu Dong-mei, Studying for master's degree, School of Stomatology, Sichuan Medical University, Luzhou 646000, Sichuan Province, China
Supported by:
the Scientific Research Project of PLA during the Twelfth Five-Year Plan, No. CWS11J024

Abstract

Abstract:

BACKGROUND: In previous experiments, we have confirmed that platelet rich fibrin has the ability of osteoinduction, and have conducted a preliminary study on its microstructure and biomechanics. However, little is reported on its histology research.
OBJECTIVE: To compare the histological changes after implanting platelet-rich fibrin, Bio-Oss and autologous bone and to analyze the pros and cons of platelet-rich fibrin implantation for repair of bone defects.
METHODS: As previously reported, animal models of critical bone defects were established respectively on the bilateral femoral condyles of 12 beagle dogs. Then, platelet-rich fibrin, Bio-Oss+collagen membrane (Bio-Oss group) and autologous bone (autologous bone group)+collagen membrane were respectively implanted. At 3, 6, 8 and 12 months, one experimental dog from each group was killed, respectively, and histological observation was performed. Another beagle dog as blank control was enrolled to establish the animal model of critical bone defects, with no implantation.
RESULTS AND CONCLUSION: At 3, 6, 8 and 12 months after implantation, there were significant differences in the new bone formation speed and amount between the platelet-rich fibrin group, Bio-Oss group and autologous bone group. These three kinds of bone grafts all had osteoinductive ability to different extents. In the platelet-rich fibrin group, the osteogenic effects were better at 3 and 6 months, and the new bone was similar to natural one; in the autologuos bone group, bone necrosis was noticeable at 3 and 6 months, but the osteogenic effects became better at 8 months, and the new bone was similar to natural one at 12 months; in the Bio-oss group, the osteogenic effects were similar to those in the platelet-rich fibrin group, but the residual of Bio-oss was visible at 12 months; in the blank control group, no bone formed at 3 months, indicating the animal model of critical bone defects was made successfully. In brief, the platelet-rich fibrin has good osteoinductive ability, with shorter time and better quality.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Fu Dong-mei, Xiao Qiong, Yang Qin-qiu, Dong Lu, Chen Hong-liang, Sun Yong. Histological observation on new bone induced by platelet-rich fibrin[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(7): 933-939.

Figures/Tables 3

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Histological observation on new bone induced by platelet-rich fibrin

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