Changes in vimentin and vascular endothelial growth factor expression in a rat model of thin endometrium established by 95% ethanol

doi:10.3969/j.issn.2095-4344.2016.05.019

Chinese Journal of Tissue Engineering Research ›› 2016, Vol. 20 ›› Issue (5): 718-722.doi: 10.3969/j.issn.2095-4344.2016.05.019

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Changes in vimentin and vascular endothelial growth factor expression in a rat model of thin endometrium established by 95% ethanol

Xu Yan-ping¹, Zhao Gui-feng², Miao Jia-ning², Tan Ji-chun¹

¹Center for Assisted Reproduction, Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University, Shenyang 110001, Liaoning Province, China; ²Experimental Research Center, Shengjing Hospital of China Medical University, Benxi 117000, Liaoning Province, China

Received:2015-12-16 Online:2016-01-29 Published:2016-01-29
Contact: Tan Ji-chun, M.D., Chief physician, Professor, Doctoral supervisor, Center for Assisted Reproduction, Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University, Shenyang 110001, Liaoning Province, China
About author:Xu Yan-ping, Studying for master’s degree, Center for Assisted Reproduction, Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University, Shenyang 110001, Liaoning Province, China
Supported by:
the Liaoning Province Science and Technology Program, No. 2012225014

Abstract

Abstract:

BACKGROUND: Ethanol chemical damage method can be used to establish thin endometrium rat model. Expression of proliferation-related proteins is decreased in thin endometrium animal model, which contributes to the study of thin endometrium-related mechanism and provides guidance for clinical treatment.
OBJECTIVE: To explore the changes in vimentin and vascular endothelial growth factor expression in rat models of high concentrations of ethanol-induced thin endometrium.
METHODS: 0.3 mL 95% ethanol was slowly injected in the right uterus of female Sprague-Dawley rats to establish rat models of thin endometrium. An equal volume of physiological saline was injected into the left uterus as control sides. RESULTS AND CONCLUSION: The thickness of endometrium in the injured uterus was significantly thinner than the control ones. Number of glands reduced, structure became disorder, and vimentin and vascular endothelial growth factor expression markedly diminished. These results suggest that 95% ethanol injury can effectively establish rat models of thin endometrium. Proliferation-related protein expression altered in the thin endometrium.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Xu Yan-ping, Zhao Gui-feng, Miao Jia-ning, Tan Ji-chun. Changes in vimentin and vascular endothelial growth factor expression in a rat model of thin endometrium established by 95% ethanol[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(5): 718-722.

Figures/Tables 2

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Changes in vimentin and vascular endothelial growth factor expression in a rat model of thin endometrium established by 95% ethanol

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