Preparation of polylactic acid/bioactive glass composite material by vacuum freeze-drying technique and study on its performance

doi:10.3969/j.issn.2095-4344.1435

Abstract

Abstract:

BACKGROUND: Bioactive glass added into polylactic acid can improve the mechanical property of the material.

OBJECTIVE: To prepare the tissue-engineered scaffold of polylactic acid/bioactive glass composites by vacuum freeze-drying technique and study its performance.

METHODS: For 1,4-dioxane and dichloromethane as pore-forming agents, polylactic acid containing 10%, 20% and 30% bioactive glass were prepared by vacuum freeze-drying technique. The porosity and compressive strength were detected. In order to observe the microstructure and element changes of composites before and after soaked into the simulated body fluid for 2 weeks. L929 fibroblasts were cultured with polylactic acid leach liquor, polylactic acid/bioactive glass composites leaching liquor and phenol solution, and the cells of being cultured in routine culture were used as control, respectively. After culture for 1, 3 and 5 days, MTT was used to detect cell proliferation.

RESULTS AND CONCLUSION: (1) The porosity of polylactic acid containing 10% and 20% bioactive glass was higher than that of polylactic acid (P < 0.05). (2) The compressive strength of polylactic acid/bioactive glass composite was higher than that of polylactic acid (P < 0.05), and the compressive strength of 20% bioactive glass composite was higher than that of 10% and 30% bioactive glass composites (P < 0.05). (3) Scanning electron microscopy showed that the inner pore wall of polylactic acid materials has a large amount of micropore structures, bioactive glass distributed in the composite, the pore size was uneven and the pores communicated with each other. As the increasing of bioactive glass contents, there were lots of blocked pores. After immersion in simulated body fluid for 2 weeks, polylactic acid/bioactive glass containing composites showed obvious hydroxyapatite formation, but there was no hydroxyapatite formation in polylactic acid. The immersion of polylactic acid was only the change of PH value, but the ratio of calcium and phosphorus was higher than that before soaking in composite materials. After soaking, there was a large amount of hydroxyapatite formation, and the proportion of calcium and phosphorus was reduced accordingly. At the same time, the Si in bioactive glass was released. (4) The cell proliferation in the polylactic acid/bioactive glass composite containing 10% and 20% bioactive glass was significantly faster than that of positive control group after 1, 3 and 5 days of culture (P < 0.05), which showed no significant difference compared with the negative control and polylactic acid groups (P > 0.05) (5) These results suggest that polylactic acid/bioactive glass containing composite scaffolds with good porosity, compressive strength and cellular compatibility can be prepared by vacuum freeze-drying technique.

Key words: polylactic acid/bioactive glass containing composite material, bioactive glass, polylactic acid, vacuum freeze-drying technique, bone tissue-engineered scaffold material, porosity, compressive strength, cell proliferation

CLC Number:

Ma Lijuan, Deng Jiupeng, Yin Haoyue, Tian Yiwen. Preparation of polylactic acid/bioactive glass composite material by vacuum freeze-drying technique and study on its performance[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(30): 4793-4798.

Figures/Tables 5

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