Growth differentiation factor 5 induces adipose-derived stem cells differentiating into chondrocytes

doi:10.3969/j.issn.2095-4344.2016.51.004

Abstract

Abstract:

BACKGROUND: Growth differentiation factor 5 (GDF5) has been shown to play a crucial role in the development of chondrocytes via different regulatory mechanisms.
OBJECTIVE: To explore the effect of GDF5 on the chondrogenic differentiation of adipose-derived stem cells (ADSCs).
METHODS: ADSCs in passage 4 isolated from Japanese White rabbits were cultured in the medium containing 0 (blank control group), 10, 50, 100, 150 and 200 μg/L, respectively. The morphological changes were observed, and the optimal concentration of GDF-5 was screened, and its round coverslips at 1, 2 and 3 weeks of culture were selected to undergo immunocytochemistry, toluidine blue staining and immunofluorescent staining.
RESULTS AND CONCLUSION: The growth of ADSCs was stable in the medium containing 10 and 50 μg/L GDF5, while apoptotic cells appeared after induction with 200 μg/L GDF5. In the medium containing 100 and 150 μg/L GDF5, some spindle-shaped cells changed into irregular shape, and became round or oval after 7-10 days of culture. The optimal concentration of GDF5 was 100 μg/L. The cells transfected by 100 μg/L GDF5 were positive for type II collagen obviously and with blue-stained nucleus. The transfected cells were positive for toluidine blue, and metachromatic granules were visible in the cytoplasm. The proteoglycan mRNA expression of the transfected cells was significantly increased, and reached the highest at 3 weeks. These results suggest that GDF5 promotes the chondrocytic differentiation of ADSCs.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Growth Differentiation Factors, Stem Cells, Chondrocytes, Tissue Engineering

CLC Number:

R318

Li Ming-hui, Liu Yang, Sun Kai, Tan Jun-feng, Zhang Mi. Growth differentiation factor 5 induces adipose-derived stem cells differentiating into chondrocytes[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(51): 7628-7633.

Figures/Tables 1

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