C- and N-terminal telopeptides of type I collagens, osteocalcin and bone-specific alkaline phosphatase used for the early diagnosis of bone nonunion

doi:10.3969/j.issn.2095-4344.2016.51.002

Abstract

Abstract:

BACKGROUND: Detecting the serum levels of biochemical markers to assess bone fractures is simple, mini-invasive and specific. Thereafter, to predict bone nonunion by choosing an appropriate marker has become a hotspot.
OBJECTIVE: To establish an animal model of bone nonunion and explore the changing rules of the biochemical markers during the process of nonunion.
METHODS: Twenty New Zealand white rabbits aged 5-6 months were enrolled and divided into two groups. In bone defect group, a 15-mm length of bone (including the periosteum) was removed from the left mid-radius, and the medullary cavities were closed with bone wax. In bone fracture group, the mid-radius was fractured. X-ray examination was taken and blood samples were extracted preoperatively and at 2, 3, 4, 5, 6, 7, 8, 10, and 12 weeks after surgery. The serum levels of osteocalcin and bone-specific alkaline phosphatase (BSAP) as markers of bone formation, and C-terminal telopeptide of type I collagen (CTX), N-terminal telopeptide of type I collagen (NTX), and tartrate-resistant acid phosphatase 5b (TRACP 5b) as markers of bone resorption, were measured using biotin double-antibody sandwich ELISA.
RESULTS AND CONCLUSION: In the bone defect group, the bone metabolism was at a high level, suggesting that the early diagnosis of bone nonunion depends on several biochemical indicators. In the bone defect group, the serum level of CTX peaked at 5 weeks, and the serum levels of osteocalcin, BSAP and NTX decreased obviously at 4 or 5 weeks, while the serum TRACP 5b concentration did not change significantly, indicating that all above markers except TRACP 5b sensitively reflect the bone turnover in vivo. Further studies are needed to determine whether systematic monitoring of the biochemical markers can reflect the bone turnover effectively and can be used for the early diagnosis of nonunion in the rabbit model.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Fractures, Bone, Osteocalcin, Collagen Type I, Tissue Engineering

CLC Number:

R318

Wang Qing-hua, Lin Jian-ping, Shen Ning-jiang, Shi Zhan-jun, Zhou Gang, Wu Duo-neng. C- and N-terminal telopeptides of type I collagens, osteocalcin and bone-specific alkaline phosphatase used for the early diagnosis of bone nonunion[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(51): 7611-7621.

Figures/Tables 3

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