Expression of osteopontin mRNA and protein in osteoblast cell sheets cultured on different titanium surfaces

doi:10.3969/j.issn.2095-4344.2017.10.005

Abstract

Abstract:

BACKGROUND: Modifying the titanium implant surface is an important method in the bone-implant osseointegration. Can the cell sheet technology realize better bone regeneration on different titanium surfaces?
OBJECTIVE: To primarily study the osteogenetic effect of osteoblast cell sheets on different titanium surfaces.
METHODS: Passage 3 osteoblasts from newborn Sprague-Dawley rats were seeded onto smooth titanium surface, anodic oxidation titanium surface, and sand-blasting/acid-etching titanium surface respectively, to make osteoblast cell sheets in vitro. At 7 and 14 days after inoculation, osteoblast cell sheets were obtained by physical scraping method and observed under inverted microscope. The expression of osteopontin mRNA and protein in osteoblasts was detected through RT-qPCR and western blot, respectively.
RESULTS AND CONCLUSION: (1) Observation with the inverted microscope: on day 7, in the gap between the titanium plate edge and the pore wall, the cells grew well in spindle shape, mutual connection matrix was found, but occasional cloud-shape cell growth was detective in dense areas close to the edge; on day 14, the growth of cells in the center area of the osteoblast cell sheets showed uniformity and overlap, the cell morphology and boundary were not clear, and the edge of the osteoblast cell sheets showed overlapping cloud shape. (2) Expression of osteopontin: the expression of osteopontin in osteoblast cells inoculated onto sand-blasting/acid-etching titanium surface and anodic oxidation titanium surface was significantly higher than that on the smooth titanium surface (P < 0.05); however, the expression of osteopontin in osteoblasts inoculated onto anodic oxidation titanium surface and sand-blasting/ acid-etching titanium surface had no statistically significant difference. The expression of osteopontin in osteoblasts on different titanium surfaces inoculated on day 14 was significantly higher than that on day 7 (P < 0.05). To conclude, sand-blasting/acid-etching titanium surface and anodic oxidation titanium surface play more significant role in promoting osteoblasts proliferation and differentiation than the smooth titanium surface.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Titanium, Osteoblasts, Osteopontin, Tissue Engineering

CLC Number:

R318

Wu Lei, Xia Qian, Mao Jiu-feng, Guo Yi, Zeng Xiao, Li Zhi, Wang Bi-chao, Dong Qiang. Expression of osteopontin mRNA and protein in osteoblast cell sheets cultured on different titanium surfaces[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(10): 1501-1507.

Figures/Tables 5

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